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1
Generation of T-helper cells in vitro. II. Analysis of supernates derived from T-helper cell cultures.体外T辅助细胞的生成。II. 对T辅助细胞培养上清液的分析。
J Exp Med. 1977 Mar 1;145(3):693-708. doi: 10.1084/jem.145.3.693.
2
Generation of T-helper cells in vitro. I. Cellular and antigen requirements.体外T辅助细胞的生成。I. 细胞和抗原需求。
J Exp Med. 1977 Mar 1;145(3):676-92. doi: 10.1084/jem.145.3.676.
3
Regulation of anti-hapten antibody responses in vivo: memory, carrier-specific Lyt-2+ T cells can terminate antibody production without altering the peak of response.体内抗半抗原抗体反应的调节：记忆性、载体特异性Lyt-2⁺ T细胞可终止抗体产生而不改变反应峰值。
J Immunol. 1984 Apr;132(4):1639-46.
4
Effects of hapten epitope structure and hapten-self conjugation pattern on T cell specificity and Ir gene control in hapten-self cytotoxic and helper T cell responses.半抗原表位结构和半抗原-自身缀合模式对半抗原-自身细胞毒性和辅助性T细胞应答中T细胞特异性及Ir基因控制的影响。
J Immunol. 1984 Jan;132(1):57-62.
5
Characterization of a concanavalin A supernatant-derived idiotype-specific T helper cell factor.刀豆球蛋白A上清液衍生的独特型特异性T辅助细胞因子的特性分析
J Immunol. 1986 Feb 15;136(4):1295-302.
6
In vivo activity of affinity-purified helper factor from antigen-specific helper clone.来自抗原特异性辅助性克隆的亲和纯化辅助因子的体内活性
J Immunol. 1985 Mar;134(3):1717-22.
7
Activation and expansion of hapten- and protein-specific T helper cells from nonsensitized mice.从未致敏小鼠中激活并扩增半抗原特异性和蛋白质特异性辅助性T细胞。
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5625-8. doi: 10.1073/pnas.85.15.5625.
8
Genetic control of hapten-reactive helper T-cell responses and its implications for the generation of augmented antitumor cytotoxic responses.半抗原反应性辅助性T细胞应答的遗传控制及其对增强抗肿瘤细胞毒性应答产生的影响。
J Natl Cancer Inst. 1985 Jun;74(6):1269-73.
9
Functionally distinct helper T cells enriched under different culture conditions cooperate with different B cells.在不同培养条件下富集的功能不同的辅助性T细胞与不同的B细胞协同作用。
J Immunol. 1982 Nov;129(5):1843-8.
10
Major histocompatibility complex-restricted and unrestricted interactions in the T cell-dependent activation of hapten-binding B cells.主要组织相容性复合体限制和非限制的相互作用在半抗原结合B细胞的T细胞依赖性激活中。
Immunobiology. 1984 May;166(4-5):345-59. doi: 10.1016/S0171-2985(84)80013-3.

引用本文的文献

1
Partial characterization of murine and monkey helper factor to a streptococcal antigen.针对链球菌抗原的小鼠和猴辅助因子的部分特性分析。
Immunology. 1980 Nov;41(3):587-96.
2
Concanavalin A-inducible, interleukin-2-producing T cell hybridoma.伴刀豆球蛋白A诱导的、产生白细胞介素-2的T细胞杂交瘤
J Exp Med. 1980 Oct 1;152(4):893-904. doi: 10.1084/jem.152.4.893.
3
Multiple thymus-derived helper cell activities involved in antibody responses.参与抗体应答的多种胸腺来源辅助性细胞活性。
Surv Immunol Res. 1982;1(2):117-21. doi: 10.1007/BF02918334.
4
Triggering of affinity-enriched B cells. Analysis of B cell stimulation by antigen-specific helper factor or lipopolysaccharide. I. Dissection into proliferative and differentiative signals.亲和富集B细胞的触发。抗原特异性辅助因子或脂多糖对B细胞刺激的分析。I. 分解为增殖信号和分化信号。
J Exp Med. 1982 Jan 1;155(1):248-63. doi: 10.1084/jem.155.1.248.
5
Antigen-specific T-cell factors.抗原特异性T细胞因子。
Immunology. 1980 Dec;41(4):759-87. doi: 10.1007/BF00199524.
6
Antigen specific T cell factors.抗原特异性T细胞因子。
Mol Cell Biochem. 1980 May 7;30(3):177-93. doi: 10.1007/BF00230172.
7
Nature of T-cell macrophage interaction in helper-cell induction in vitro. II. Two stages of T-helper-cell differentiation analyzed in irradiation and allophenic chimeras.体外辅助性T细胞诱导过程中T细胞与巨噬细胞相互作用的性质。II. 在辐射嵌合体和异表型嵌合体中分析辅助性T细胞分化的两个阶段。
J Exp Med. 1979 Mar 1;149(3):686-701. doi: 10.1084/jem.149.3.686.
8
The role of H-2 linked genes in helper T-cell function. IV. Importance of T-cell genotype and host environment in I-region and Ir gene expression.H-2连锁基因在辅助性T细胞功能中的作用。IV. T细胞基因型和宿主环境在I区及Ir基因表达中的重要性。
J Exp Med. 1978 Dec 1;148(6):1510-22. doi: 10.1084/jem.148.6.1510.
9
Thymus dependence of the IgG response: role of T cells is restricted to non-specific rather than antigen-specific factors.IgG应答的胸腺依赖性：T细胞的作用仅限于非特异性而非抗原特异性因素。
Immunology. 1979 Jul;37(3):603-8.

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Chemical coupling of proteins to agarose.蛋白质与琼脂糖的化学偶联。
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Cell-to-cell interaction in the immune response. VII. Requirement for differentiation of thymus-derived cells.免疫反应中的细胞间相互作用。VII. 胸腺衍生细胞分化的必要性。
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Reconstitution of a thymus cell-deprived immune system by syngeneic and allogeneic thymocytes in vitro.通过同基因和异基因胸腺细胞在体外重建胸腺细胞缺失的免疫系统。
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Cell interactions in the immune response in vitro. IV. Comparison of the effects of antigen-specific and allogeneic thymus-derived cell factors.体外免疫应答中的细胞相互作用。IV. 抗原特异性和同种异体胸腺衍生细胞因子作用的比较。
J Exp Med. 1972 Oct 1;136(4):722-36. doi: 10.1084/jem.136.4.722.
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Inhibition of in vitro immune response by treatment of spleen cell suspensions with anti-theta serum.用抗θ血清处理脾细胞悬液对体外免疫反应的抑制作用。
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The role of antibody in T-cell responses.抗体在T细胞应答中的作用。
Clin Exp Immunol. 1974 May;17(1):1-18.
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Antigen-specific T-cell factor in cell cooperation: physical properties and mapping in the left-hand (K) half of H-2.细胞协作中的抗原特异性T细胞因子：物理性质及在H-2左手（K）半区的定位
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Cooperation by mouse T lymphocytes: the role of antibody in T cell specificity.小鼠T淋巴细胞的合作：抗体在T细胞特异性中的作用。
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体外T辅助细胞的生成。II. 对T辅助细胞培养上清液的分析。

Generation of T-helper cells in vitro. II. Analysis of supernates derived from T-helper cell cultures.

作者信息

McDougal J S, Gordon D S

机构信息

Parasitology Division, Center for Disease Control, Atlanta, Georgia 30333.

出版信息

J Exp Med. 1977 Mar 1;145(3):693-708. doi: 10.1084/jem.145.3.693.

DOI:10.1084/jem.145.3.693
PMID:233920
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2180701/
Abstract

Supernates derived from in vitro generated T-helper cells have been analyzed for their capacity to substitute for T-cell carrier reactivity. T-helper cell supernates stimulate both a carrier-specific and nonspecific anti-DNP-PFC response to DNP-carrier conjugates in cultures of hapten-primed spleen cells. The carrier-specific and nonspecific activity can be distinguished by dosage optimum, antigen requirements, binding specificity for carrier, and in the requirement for additional splenic adherent accessory cell involvement. The active factors produced in this system are heat labile and sensitive to trypsin and periodate. They are removed by absorption with alloantisera directed toward the strain from which the supernate was derived but not by a variety of anti-immunoglobulin sera.

摘要

已对源自体外产生的辅助性T细胞的上清液替代T细胞载体反应性的能力进行了分析。在半抗原致敏的脾细胞培养物中，辅助性T细胞上清液可刺激对DNP-载体缀合物的载体特异性和非特异性抗DNP-PFC反应。载体特异性和非特异性活性可通过最佳剂量、抗原需求、对载体的结合特异性以及对额外脾黏附辅助细胞参与的需求来区分。该系统中产生的活性因子对热不稳定，对胰蛋白酶和高碘酸盐敏感。它们可通过用针对上清液来源品系的同种抗血清吸收而去除，但不能被多种抗免疫球蛋白血清去除。