Ockner R K, Manning J A, Poppenhausen R B, Ho W K
Science. 1972 Jul 7;177(4043):56-8. doi: 10.1126/science.177.4043.56.
A protein of molecular weight approximately 12,000 which binds long-chain fatty acids and certain other lipids has been identified in cytosol of intestinal mucosa, liver, myocardium, adipose tissue, and kidney. Binding is noncovalent and is greater for unsaturated than for saturated and medium-chain fatty acids. This protein appears to be identical with the smaller of two previously described cytoplasmic anion-binding proteins. Binding of long-chain fatty acids by this protein is greater than that of other anions tested, including sulfobromophthalein, and does not depend on negative charge alone. The presence of this binding protein may explain previously observed differences in intestinal absorption among fatty acids, and the protein may participate in the utilization of long-chain fatty acids by many mammalian tissues.
在肠黏膜、肝脏、心肌、脂肪组织和肾脏的胞质溶胶中，已鉴定出一种分子量约为12,000的蛋白质，它能结合长链脂肪酸和某些其他脂质。这种结合是非共价的，对不饱和脂肪酸的结合力大于对饱和脂肪酸和中链脂肪酸的结合力。该蛋白质似乎与先前描述的两种细胞质阴离子结合蛋白中较小的一种相同。这种蛋白质对长链脂肪酸的结合力大于所测试的其他阴离子，包括磺溴酞，且不仅仅取决于负电荷。这种结合蛋白的存在可能解释了先前观察到的脂肪酸在肠道吸收方面的差异，并且该蛋白质可能参与许多哺乳动物组织对长链脂肪酸的利用。
