钙离子与细胞融合。化学融合剂对红细胞钙及其他离子通透性的影响。
Calcium ions and cell fusion. Effects of chemical fusogens on the permeability of erythrocytes to calcium and other ions.
作者信息
Blow A M, Botham G M, Lucy J A
出版信息
Biochem J. 1979 Aug 15;182(2):555-63. doi: 10.1042/bj1820555.
Abstract
- Fusogenic and non-fusogenic chemicals were tesetd for their ability to allow 45Ca2+ and 3H2O to enter hen and human erythrocytes. 2. The ratio of 45Ca2+/3H2O in treated cells to that in untreated cells is referred to as the entry ratio. 3. Within 1 min at 37 degrees C both water-soluble and lipid-soluble fusogens increased the value of the entry ratio, which reached maximum values in 5--10 min. 4. Values of the entry ratio in the range of 4--12 were found under conditions that led to cell fusion. 5. Closely related but non-fusogenic chemicals did not significantly alter the entry ratio. 6. The entry ratios for 86Rb+, 22Na+ and 35SO42- were also significantly increased by both lipid-soluble and water-soluble fusogens, though the increases were not as large as those for 45Ca2+. 7. It is suggested that fusogenic compounds increase the permeability of biological membranes to ions, and that an increase in the concentration of intracellular Ca2+ initiates or facilitates events that lead to the chemically induced fusion of erythrocytes.
摘要
- 对促融合和非促融合化学物质进行了测试，以检测它们使45Ca2+和3H2O进入母鸡和人类红细胞的能力。2. 处理过的细胞中45Ca2+/3H2O与未处理细胞中的该比值被称为进入率。3. 在37摄氏度下1分钟内，水溶性和脂溶性促融合剂均提高了进入率的值，该值在5至10分钟内达到最大值。4. 在导致细胞融合的条件下，发现进入率的值在4至12范围内。5. 密切相关但非促融合的化学物质不会显著改变进入率。6. 脂溶性和水溶性促融合剂也显著提高了86Rb+、22Na+和35SO42-的进入率，尽管提高幅度不如45Ca2+的大。7. 有人提出，促融合化合物会增加生物膜对离子的通透性，并且细胞内Ca2+浓度的增加会引发或促进导致化学诱导红细胞融合的事件。
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9
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本文引用的文献
1
Studies on the mode of action of excess of vitamin A. 5. The effect of vitamin A on the stability of the erythrocyte membrane.过量维生素A作用机制的研究。5. 维生素A对红细胞膜稳定性的影响。
Biochem J. 1962 Sep;84(3):611-21. doi: 10.1042/bj0840611.
2
The rate of exchange of tritiated water across the human red cell membrane.氚标记水穿过人红细胞膜的交换速率。
J Gen Physiol. 1957 Nov 20;41(2):259-77. doi: 10.1085/jgp.41.2.259.
3
Properties of ionophores with broad range cation selectivity.具有广泛阳离子选择性的离子载体的特性。
Fed Proc. 1973 Jun;32(6):1698-703.
4
The fusion of erythrocytes by fatty acids, esters, retinol and alpha-tocopherol.红细胞与脂肪酸、酯类、视黄醇和α-生育酚的融合。
Biochem J. 1973 Sep;136(1):147-55. doi: 10.1042/bj1360147.
5
Relationships between the exchange of calcium and phosphate in isolated fat-cells.分离脂肪细胞中钙与磷酸盐交换的关系。
Biochem J. 1975 Oct;152(1):121-9. doi: 10.1042/bj1520121.
6
Studies on mixed monolayers of phospholipids and fusogenic lipids.磷脂与促融脂质混合单分子层的研究。
Biochem J. 1975 Sep;149(3):597-608. doi: 10.1042/bj1490597.
7
Mechanisms of cell fusion.细胞融合的机制。
Nature. 1975 Jan 17;253(5488):194-5. doi: 10.1038/253194a0.
8
Promotion of cell fusion by divalent cation ionophores.二价阳离子离子载体对细胞融合的促进作用。
Nature. 1975 Jul 17;256(5514):208-9. doi: 10.1038/256208a0.
9
Fusion of hen erythrocytes with yeast protoplasts induced by polyethylene glycol.聚乙二醇诱导鸡红细胞与酵母原生质体融合。
Nature. 1975 May 1;255(5503):66-7. doi: 10.1038/255066a0.
10
Poly(ethylene glycol), surface potential and cell fusion.聚乙二醇、表面电位与细胞融合
Biochem J. 1976 Sep 15;158(3):647-50. doi: 10.1042/bj1580647.
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