纯化的聚乙二醇能诱导细胞融合吗？

Is purified poly(ethylene glycol) able to induce cell fusion?

作者信息

Smith C L, Ahkong Q F, Fisher D, Lucy J A

出版信息

Biochim Biophys Acta. 1982 Oct 22;692(1):109-14. doi: 10.1016/0005-2736(82)90508-9.

Abstract

Preparations of poly(ethylene glycol) 6000 (PEG) from five different commercial sources have been purified, and their ability to fuse hen erythrocytes has been investigated. Quantitative assessments of cell fusion showed that before purification one of the preparations (PEG Wako), was able to induce limited fusion (5-6%) of erythrocytes with conditions (1 min incubation with 50% w/w PEG) under which the other four unpurified preparations of PEG were inactive. On purification, PEG (Wako) became inactive. By contrast, when erythrocytes were incubated with 45% w/w PEG for 15 min, extensive fusion (23-27%) occurred with all five unpurified preparations of PEG. Under these conditions, the fusogenic properties of four of the preparations of PEG were unaffected by purification: fusion induced by PEG (Wako) was, however, decreased on purification from 27% to 19%. It appears that polymeric poly(ethylene glycol) is itself able to fuse cells, but that some commercial preparations, e.g. PEG (Wako), have enhanced fusogenic properties resulting from the presence of contaminating substances. No relationship between the absorbance at 290 nm of PEG and its fusogenic properties was found in this study. The addition of small quantities of fusogenic lipid-soluble compounds to PEG was, however, observed to enhance cell fusion by up to 50%.

摘要

对来自五个不同商业来源的聚乙二醇6000（PEG）制剂进行了纯化，并研究了它们使鸡红细胞融合的能力。细胞融合的定量评估表明，在纯化之前，一种制剂（PEG和光纯药）在其他四种未纯化的PEG制剂无活性的条件下（在50% w/w PEG中孵育1分钟）能够诱导有限的红细胞融合（5-6%）。纯化后，PEG（和光纯药）变得无活性。相比之下，当红细胞在45% w/w PEG中孵育15分钟时，所有五种未纯化的PEG制剂都发生了广泛的融合（23-27%）。在这些条件下，四种PEG制剂的促融合特性不受纯化影响：然而，PEG（和光纯药）诱导的融合在纯化后从27%降至19%。看来聚合聚乙二醇本身能够使细胞融合，但一些商业制剂，如PEG（和光纯药），由于污染物的存在而具有增强的促融合特性。在本研究中未发现PEG在290 nm处的吸光度与其促融合特性之间的关系。然而，观察到向PEG中添加少量促融合脂溶性化合物可使细胞融合增强高达50%。