Troy F A, Fenyö E M, Klein G
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5270-4. doi: 10.1073/pnas.74.12.5270.
An experimental procedure for detecting and characterizing tumor-associated, virion, and histocompatibility antigens has been developed. The method takes advantage of the high resolution that proteins, solubilized by Triton X-100 and reduced, display after sodium dodecyl sulfate gel electrophoresis. The antigens can be detected as distinct molecular weight species by a highly sensitive inhibition of cytotoxic reaction. When coupled to the lactoperoxidase-catalyzed iodination of intact cells, the procedure permits the determination of externally exposed antigens. In the present study, the method has been applied to the Moloney leukemia virus-induced YAC lymphoma cells of strain A mice, which express a Moloney leukemia virus-determined cell surface antigen (MCSA) in addition to the type C viral proteins gp71, p30, p15, p15(E), p12, and p10. MCSA was identified as an exposed surface protein distinct in size and antigenic determinants from the major envelope and core protein of Moloney leukemia virus and the histocompatibility antigens. Multiple molecular weight species possessing antigenic determinants for MCSA, gp71, and H-2(a) have been detected. These results provide direct confirmation that MCSA is unrelated to the known virion structural proteins or to the H-2(a) antigen. This method should permit the direct identification and molecular weight characterization of any antigen whose determinants are not solely dependent on a complex quaternary structure and for which serological reagents are available.
已开发出一种用于检测和表征肿瘤相关抗原、病毒体抗原和组织相容性抗原的实验方法。该方法利用了经Triton X - 100溶解并还原的蛋白质在十二烷基硫酸钠凝胶电泳后所显示的高分辨率。通过对细胞毒性反应的高度敏感抑制，可将抗原检测为不同分子量的物质。当与完整细胞的乳过氧化物酶催化碘化反应相结合时，该方法可用于测定细胞表面暴露的抗原。在本研究中，该方法已应用于莫洛尼白血病病毒诱导的A系小鼠YAC淋巴瘤细胞，这些细胞除了表达C型病毒蛋白gp71、p30、p15、p15(E)、p12和p10外，还表达一种由莫洛尼白血病病毒决定的细胞表面抗原（MCSA）。MCSA被鉴定为一种暴露的表面蛋白，其大小和抗原决定簇与莫洛尼白血病病毒的主要包膜和核心蛋白以及组织相容性抗原不同。已检测到具有MCSA、gp71和H - 2(a)抗原决定簇的多种分子量物质。这些结果直接证实了MCSA与已知的病毒体结构蛋白或H - 2(a)抗原无关。该方法应能直接鉴定任何其抗原决定簇不 solely 依赖于复杂四级结构且有血清学试剂可用的抗原，并对其进行分子量表征。 （注：原文中“solely”拼写错误，正确应为“solely”，翻译时已修正）
