Rigdon R H, Schadewald T
Appl Microbiol. 1972 Oct;24(4):634-7. doi: 10.1128/am.24.4.634-637.1972.
Freund complete adjuvant, with allegedly "killed and dried" Mycobacterium butyricum added (intramuscularly in rabbits and intraperitoneally in mice), produced a local granulomatous lesion and frequently a similar lesion in the viscera. Acid-fast bacilli were present in these lesions, and in some animals M. butyricum was obtained in culture. When this culture was inoculated in mice, a similar lesion developed. Pathological lesions with similar acid-fast organisms also were observed by us in rabbits given Freund complete adjuvant by two other investigators.
弗氏完全佐剂添加了据称“已杀死并干燥”的丁酸分枝杆菌（在兔子中肌肉注射，在小鼠中腹腔注射），会产生局部肉芽肿病变，且在内脏中常常出现类似病变。这些病变中存在抗酸杆菌，在一些动物中，培养得到了丁酸分枝杆菌。将这种培养物接种到小鼠中时，会出现类似病变。另外两名研究人员给兔子注射弗氏完全佐剂后，我们在兔子身上也观察到了带有类似抗酸菌的病理病变。
