绵羊线粒体DNA的分子特征分析与克隆

Molecular characterization and cloning of sheep mitochondrial DNA.

作者信息

Villalta M, Fernández-Silva P, Beltrán B, Enguita L, López-Pérez M J, Montoya J

机构信息

Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Spain.

出版信息

Curr Genet. 1992 Mar;21(3):235-40. doi: 10.1007/BF00336847.

DOI:10.1007/BF00336847

PMID:1563049

Abstract

Mitochondrial DNA from the liver of a single Rasa Aragonesa sheep has been isolated and characterized. The size of the genome, determined by restriction enzyme analysis, was found to be 16.58 kbp. The cleavage sites for the restriction endonucleases BamHI, HindIII, EcoRI, BglII, PvuII, BstEII and PstI were mapped, and the gene organization deduced through heterologous hybridization using different cloned fragments of the rat mitochondrial genome. Fragments representative of the entire sheep genome were cloned in plasmid vectors pGEM3Z and pUN121.

摘要

已从一只阿拉贡拉莎绵羊的肝脏中分离并鉴定出线粒体DNA。通过限制性内切酶分析确定的基因组大小为16.58千碱基对。绘制了限制性内切酶BamHI、HindIII、EcoRI、BglII、PvuII、BstEII和PstI的切割位点，并使用大鼠线粒体基因组的不同克隆片段通过异源杂交推导基因组织。代表整个绵羊基因组的片段被克隆到质粒载体pGEM3Z和pUN121中。