黄曲霉毒素对离体肝细胞摄取RNA和蛋白质前体的影响。
The effect of aflatoxins on the incorporation of RNA and protein precursors by isolated hepatocytes.
作者信息
McIntosh P R, Evans I H, Rabin B R
出版信息
Br J Cancer. 1976 Apr;33(4):440-9. doi: 10.1038/bjc.1976.69.
Hepatocytes prepared by a simplified enzymatic technique were active in the incorporation of RNA and protein precursors into acid-insoluble material. The incorporation of RNA precursors was very markedly inhibited by low levels of aflatoxin B1 and G1 but not by aflatoxins B2 and G2. The activity of mixed function oxidases (MFO), the drug-metabolizing system of the endoplasmic reticulum, could be suppressed in these cells by SKF525A or stimulated by NADPH. SKF525A caused a reduction in the inhibition by aflatoxin B1 of the incorporation of RNA precursor into macromolecules. This finding suggests that a metabolite of aflatoxin B1 is the actual inhibitor of RNA synthesis in the cells. Measurement of lactate dehydrogenase activity showed these cells to be leaky on incubation at 37 degrees C and thus not suitable for studies of protein secretion.
通过简化酶技术制备的肝细胞能够将RNA和蛋白质前体掺入酸不溶性物质中。低水平的黄曲霉毒素B1和G1能非常显著地抑制RNA前体的掺入，但黄曲霉毒素B2和G2则无此作用。内质网的药物代谢系统——混合功能氧化酶（MFO）的活性，在这些细胞中可被SKF525A抑制或被NADPH刺激。SKF525A可减少黄曲霉毒素B1对RNA前体掺入大分子的抑制作用。这一发现表明，黄曲霉毒素B1的一种代谢产物是细胞中RNA合成的实际抑制剂。乳酸脱氢酶活性的测定表明，这些细胞在37℃孵育时会渗漏，因此不适合用于蛋白质分泌的研究。
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