Schott H, Eckstein H
Hoppe Seylers Z Physiol Chem. 1979 Nov;360(11):1689-92.
Homologues of deoxyriboadenylic and deoxyribothymidylic acid show different degrees of retardation in a pentalysine gel. This chromatographic retardation is directly related to the strength of the interaction between the oligonucleotides and the immobilized pentalysine peptide residues. The strength of this interaction is determined by the total negative charge of the oligonucleotide, and by the nature of the constituent base of the oligonucleotide. The specificity of the interaction depends upon differences in conformation of the oligonucleotides.
脱氧核糖腺苷酸和脱氧核糖胸苷酸的同系物在五赖氨酸凝胶中表现出不同程度的滞留。这种色谱滞留与寡核苷酸和固定化五赖氨酸肽残基之间相互作用的强度直接相关。这种相互作用的强度由寡核苷酸的总负电荷以及寡核苷酸组成碱基的性质决定。相互作用的特异性取决于寡核苷酸构象的差异。
