Brantl V, Teschemacher H, Henschen A, Lottspeich F
Hoppe Seylers Z Physiol Chem. 1979 Sep;360(9):1211-6. doi: 10.1515/bchm2.1979.360.2.1211.
A material which displayed opioid activity in the guinea pig ileum longitudinal muscle-myenteric plexus preparation was extracted from an enzymatic casein digest into chloroform/methanol. The extract was roughly purified by adsorption/desorption procedures using charcoal and Amberlite XAD-2 resin as adsorbents. A high degree of purity was achieved by high-pressure liquid chromatography of the material on muBondapak C18 and mu-Porasil columns and finally by gel filtration chromatography on a Bio-Gel P-2 column. Several pronase-resistant compounds with opioid activity were obtained.
从酶解酪蛋白消化物中提取出一种在豚鼠回肠纵肌-肠肌丛制备物中表现出阿片样活性的物质，将其提取到氯仿/甲醇中。提取物通过以活性炭和Amberlite XAD-2树脂为吸附剂的吸附/解吸程序进行粗纯化。通过在μBondapak C18和μ-Porasil柱上对该物质进行高压液相色谱分析，最后在Bio-Gel P-2柱上进行凝胶过滤色谱分析，达到了高纯度。获得了几种具有阿片样活性的抗链霉蛋白酶化合物。
