一种用于测定血红蛋白A1a + b和A1c的简易微色谱柱。

A simple microchromatographic column for determination of hemoglobins A1a + b and A1c.

作者信息

Friedman S, Humbert J R

出版信息

Hemoglobin. 1979;3(6):411-28. doi: 10.3109/03630267909002279.

Abstract

A simple microchromatographic homemade column was devised for the measurement of glycosylated minor hemoglobin fractions. The mean and one standard deviation of hemoglobins A1a + b, A1c and A1a + b + c determined by the homemade column in 150 nondiabetic controls and 56 juvenile onset insulin-dependent diabetics were 2.6 +/- 0.5%, 6.0 +/- 1.0%, 8.6 +/- 1.1%, 3.3 +/- 0.9%, 13.7 +/- 2.2% and 16.9 +/- 2.8% respectively. A twofold increase in hemoglobins A1c and A1a + b + c levels was observed in the diabetics as compared to the non-diabetic controls suggesting that the homemade column is valid for the measurement of all three glycosylated hemoglobin fractions and assessment of blood glucose control in diabetic patients. The homemade column procedure yields accurate and reproducible results, is simple to perform, inexpensive, relatively rapid and may be used in the routine clinical laboratory. Hemoglobin A1a + b + c levels measured by a commercial column in 35 non-diabetic controls and in 56 diabetics showed good correlation (R = 0.91) with hemoglobin A1a + b + c levels determined by the homemade column. The commercial column is valid for the measurement of the combined glycosylated hemoglobin A1a + b + c fraction and may be used in the routine clinical laboratory to assess diabetic control.

摘要

设计了一种简单的自制微型色谱柱用于测定糖化血红蛋白亚组分。在150名非糖尿病对照者和56名青少年起病的胰岛素依赖型糖尿病患者中，用自制柱测定的血红蛋白A1a + b、A1c以及A1a + b + c的均值和一个标准差分别为2.6 +/- 0.5%、6.0 +/- 1.0%、8.6 +/- 1.1%、3.3 +/- 0.9%、13.7 +/- 2.2%和16.9 +/- 2.8%。与非糖尿病对照者相比，糖尿病患者的血红蛋白A1c和A1a + b + c水平增加了两倍，这表明自制柱可有效测定所有三种糖化血红蛋白亚组分，并可用于评估糖尿病患者的血糖控制情况。自制柱法结果准确、可重复，操作简单、成本低廉、相对快速，可用于常规临床实验室。用商业柱在35名非糖尿病对照者和56名糖尿病患者中测定的血红蛋白A1a + b + c水平与用自制柱测定的血红蛋白A1a + b + c水平具有良好的相关性（R = 0.91）。商业柱可有效测定糖化血红蛋白A1a + b + c的组合亚组分，可用于常规临床实验室评估糖尿病控制情况。