Hirschberg R, Ensign J C
J Bacteriol. 1971 Nov;108(2):757-9. doi: 10.1128/jb.108.2.757-759.1971.
Three types of evidence are presented to show that the enzymes that hydroxylate nicotinic acid to 2,6-dihydroxynicotinic acid use water as a source of oxygen atoms. (18)O is incorporated into the products from H(2) (18)O. Molecular oxygen acts as a terminal electron acceptor, one-half molecule being consumed per molecule of hydroxyl groups incorporated. An external electron acceptor is required for activity in purified preparations.
本文提供了三类证据，以表明将烟酸羟基化为2,6 - 二羟基烟酸的酶以水作为氧原子来源。（18）O从H₂（18）O掺入产物中。分子氧作为末端电子受体，每掺入一分子羟基消耗二分之一分子的氧。在纯化制剂中，活性需要外部电子受体。
