Randolph W C, Osborne V L, Intoccia A P
J Pharm Sci. 1979 Nov;68(11):1451-3. doi: 10.1002/jps.2600681131.
A method is described for the extraction of ticrynafen, a new hypotensive agent, and its reduced metabolite from serum and urine. Drug-related material is extracted from biological fluids with ether under strongly acidic conditions and then back-extracted into an alkaline aqueous phase, which is subjected to high-pressure liquid chromatographic analysis. Separations are performed on a reversed-phase column with a mobile phase consisting of phosphate buffer-acetonitrile. This accurate and reproducible method measures serum concentrations of ticrynafen and its reduced metabolite as low as 1.0 and 0.4 microgram/ml, respectively.
本文描述了一种从血清和尿液中提取新型降压药替克瑞纳芬及其还原代谢物的方法。在强酸性条件下用乙醚从生物流体中提取与药物相关的物质，然后反萃取到碱性水相中，再进行高压液相色谱分析。分离在反相柱上进行，流动相由磷酸盐缓冲液 - 乙腈组成。这种准确且可重复的方法可分别测定低至1.0微克/毫升和0.4微克/毫升的替克瑞纳芬及其还原代谢物的血清浓度。
