用气相色谱法测定人及动物尿液、血清和血浆中替尼酸及其代谢物。

GLC determination of ticrynafen and its metabolites in urine, serum, and plasma of humans and animals.

作者信息

Hwang B, Konicki G, Dewey R, Miao C

出版信息

J Pharm Sci. 1978 Aug;67(8):1095-8. doi: 10.1002/jps.2600670818.

Abstract

A sensitive GLC assay for ticrynafen, a diuretic agent with uricosuric properties, and its two metabolites in urine, serum, and plasma is described. The method employs methylation of carboxylic acid groups and trimethylsilyation of the hydroxyl group on one metabolite that cannot otherwise be separated readily from ticrynafen as a simple methyl ester. Urinary output and serum or plasma levels of ticrynafen and its two metabolites were measured in specimens from human volunteers receiving one 250-mg tablet.

摘要

本文描述了一种灵敏的气相色谱法，用于测定具有促尿酸排泄特性的利尿药替尼酸及其在尿液、血清和血浆中的两种代谢产物。该方法采用羧酸基团甲基化以及一种代谢产物上羟基的三甲基硅烷化，否则该代谢产物无法作为简单甲酯与替尼酸轻易分离。在接受一片250毫克片剂的人类志愿者的样本中，测定了替尼酸及其两种代谢产物的尿量、血清或血浆水平。