Wright J F, Kurosky A, Pryzdial E L, Wasi S
Canadian Red Cross Society, Toronto Centre.
J Virol. 1995 Aug;69(8):4784-91. doi: 10.1128/JVI.69.8.4784-4791.1995.
A significant amount of host cellular annexin II was found to be associated with human cytomegalovirus isolated from cultured human fibroblasts (approximately 1,160 molecules per virion). This composition was established by four different analytical approaches that included (i) Western blot (immunoblot) analysis of gradient-purified virions with a monoclonal antibody specific for annexin II, (ii) peptide mapping and sequence analysis of virus-associated proteins and proteins dissociated from virus following EDTA treatment, (iii) electron microscopic immunocytochemistry of gradient-purified virions, and (iv) labeling of virus-associated proteins by lactoperoxidase-catalyzed radioiodination. These results indicated that annexin II was primarily localized to the viral surface, where it bound in a divalent cation-dependent manner. In functional experiments, a rabbit antiserum raised against annexin II inhibited cytomegalovirus plaque formation in human foreskin fibroblast monolayers in a concentration-dependent manner. Cumulatively, these studies demonstrate an association of host annexin II with cytomegalovirus particles and provide evidence for the involvement of this cellular protein in virus infectivity.
发现大量宿主细胞的膜联蛋白II与从培养的人成纤维细胞中分离出的人巨细胞病毒相关联（每个病毒体约1160个分子）。这种组成是通过四种不同的分析方法确定的，包括：（i）用对膜联蛋白II特异的单克隆抗体对梯度纯化的病毒体进行蛋白质印迹（免疫印迹）分析；（ii）对病毒相关蛋白以及经EDTA处理后从病毒上解离的蛋白进行肽图谱分析和序列分析；（iii）对梯度纯化的病毒体进行电子显微镜免疫细胞化学分析；（iv）用过氧化物酶催化的放射性碘化法对病毒相关蛋白进行标记。这些结果表明，膜联蛋白II主要定位于病毒表面，以二价阳离子依赖的方式结合在那里。在功能实验中，针对膜联蛋白II产生的兔抗血清以浓度依赖的方式抑制人包皮成纤维细胞单层中的巨细胞病毒蚀斑形成。总的来说，这些研究证明了宿主膜联蛋白II与巨细胞病毒颗粒之间的关联，并为这种细胞蛋白参与病毒感染性提供了证据。
