去甲肾上腺素在无神经支配的平滑肌中的摄取。

Noradrenaline uptake by non-innervated smooth muscle.

Burnstock G, McLean J R, Wright M

Br J Pharmacol. 1971 Sep;43(1):180-9. doi: 10.1111/j.1476-5381.1971.tb07167.x.

Abstract

Uptake of noradrenaline (NA) into the non-innervated smooth muscle cells of the human umbilical artery and the chick amnion has been studied with the fluorescence histochemical technique for localizing monoamines. Comparison has been made with uptake into sympathetically innervated smooth muscle of the rabbit ear artery.2. Accumulation of NA within non-innervated smooth muscle cells is observed histochemically after exposure to much lower concentrations of NA (10(-7) g/ml) than in sympathetically innervated smooth muscle cells, where accumulation occurs with NA (10(-5) g/ml).3. In contrast to innervated smooth muscle, uptake of NA (10(-4) g/ml) by non-innervated smooth muscle is characterized by lack of inhibition by phenoxybenzamine, normetanephrine and cold, although some inhibition is apparent at lower NA concentrations. Retention of NA during prolonged washing in NA-free Krebs demonstrates that it is strongly bound within the non-innervated smooth muscle cells, particularly in the nucleus.4. After inhibition of catechol-O-methyl transferase, the accumulation of NA in innervated smooth muscle closely resembles that in non-innervated smooth muscle.

摘要

已采用荧光组织化学技术定位单胺，对去甲肾上腺素（NA）被摄取进入人脐动脉和鸡羊膜的无神经支配平滑肌细胞的情况进行了研究。并与NA被摄取进入兔耳动脉交感神经支配平滑肌的情况作了比较。
组织化学观察到，与交感神经支配的平滑肌细胞相比，无神经支配的平滑肌细胞在接触低得多浓度的NA（10⁻⁷g/ml）后，NA会在其中蓄积，而在交感神经支配的平滑肌细胞中，NA浓度为10⁻⁵g/ml时才会发生蓄积。
与神经支配的平滑肌不同，无神经支配的平滑肌摄取NA（10⁻⁴g/ml）的特点是不受苯氧苄胺、去甲变肾上腺素和低温的抑制，不过在较低NA浓度时会有一些抑制作用。在无NA的Krebs液中长时间冲洗后NA的保留情况表明，它在无神经支配的平滑肌细胞内，尤其是在细胞核内，结合牢固。
儿茶酚-O-甲基转移酶被抑制后，神经支配的平滑肌中NA的蓄积情况与无神经支配的平滑肌中的情况极为相似。