Sinha S, Sengupta U, Ramu G, Desikan K V
Lepr India. 1979 Jul;51(3):323-9.
Four fractions each from Dharmendra and Mitsuda antigen have been obtained by step-wise centrifugation and sonication of the antigen. These fractions have been assessed for their capacity of inducing skin delayed hypersensitivity response. While, it has been noted that all fractions of both types of antigens can induce a good early reaction, the late skin reaction is only mounted by intact bacilli of both types of antigen. When compared at a constant bacillary concentration, Dharmendra antigen has produced better early skin reaction than Mitsuda antigen, whereas the intensity of late skin reaction is almost equal with both the antigens. The hypothesis has been put forward that the early, as well as the late reaction are produced by the same antigen and this antigen is located in the protoplasm of M. leprae.
通过对达曼德拉抗原和水田抗原进行逐步离心和超声处理，分别获得了四个级分。对这些级分诱导皮肤迟发型超敏反应的能力进行了评估。虽然已经注意到两种抗原的所有级分都能诱导良好的早期反应，但只有两种抗原的完整杆菌才能引发晚期皮肤反应。在杆菌浓度恒定的情况下进行比较时，达曼德拉抗原产生的早期皮肤反应比水田抗原更好，而两种抗原的晚期皮肤反应强度几乎相等。有人提出假说，早期和晚期反应是由同一抗原产生的，且该抗原位于麻风杆菌的原生质中。
