Högman C F, Arro E, Hedlund K
Haematologia (Budap). 1980;13(1-4):135-44.
Red cells stored for up to 35 days have been studied by scanning electron microscopy. Formation of spicules occurred under all storage conditions in CPD and CPD-adenine whole blood, CPD-adenine red cell concentrate, and saline-adenine-glucose red cell concentrate. The number of cells with normal or near normal shape was larger if the blood was stored as a moderately concentrated suspension in saline-adenine-glucose medium or in autologous plasma as compared to the storage as whole blood. The occurrence of echinocytes during storage at +4 degrees C was not correlated to red cell ATP. It was shown that a drastic reduction of ATP leads to an increased formation of both spherocytes and echinocytes. The change of red cell shape during storage at +4 degrees C thus can be due to two processes, one unrelated to ATP and another related to ATP. Why storage as red cell concentrate is superior is not fully understood.
已通过扫描电子显微镜对储存长达35天的红细胞进行了研究。在CPD和CPD-腺嘌呤全血、CPD-腺嘌呤红细胞浓缩液以及生理盐水-腺嘌呤-葡萄糖红细胞浓缩液的所有储存条件下，均会出现棘状突起。与全血储存相比，如果血液以中等浓度悬浮液的形式储存在生理盐水-腺嘌呤-葡萄糖培养基或自体血浆中，具有正常或接近正常形状的细胞数量会更多。在4℃储存期间棘红细胞的出现与红细胞ATP无关。结果表明，ATP的急剧减少会导致球形红细胞和棘红细胞的形成增加。因此，在4℃储存期间红细胞形状的变化可能归因于两个过程，一个与ATP无关，另一个与ATP有关。红细胞浓缩液储存为何更具优势尚不完全清楚。
