通过等电聚焦从嗜水气单胞菌中分离出两种溶血素。
Separation of two hemolysins from Aeromonas hydrophila by isoelectric focusing.
作者信息
Wretlind B, Möllby R, Wadström T
出版信息
Infect Immun. 1971 Oct;4(4):503-5. doi: 10.1128/iai.4.4.503-505.1971.
Abstract
Two hemolytic proteins (hemolysin I and II) with isoelectric points of 5.5 and 4.3 were purified by isoelectric focusing from the culture supernatant fluid of Aeromonas hydrophila. The purified hemolysins were unstable after dialysis and upon heating at 37 C and were inactivated by proteolytic enzymes. The two hemolysins were lethal for mice in a dose of 10 mug (hemolysin I) and 3 mug (hemolysin II) and showed similar hemolytic spectra on red cells of different species. Both were also cytotoxic for HeLa cells and human fibroblasts and gave dermonecrosis in rabbits.
摘要
通过等电聚焦从嗜水气单胞菌培养上清液中纯化出两种等电点分别为5.5和4.3的溶血蛋白（溶血素I和II）。纯化后的溶血素在透析后以及37℃加热时不稳定，并且会被蛋白水解酶灭活。这两种溶血素对小鼠的致死剂量分别为10微克（溶血素I）和3微克（溶血素II），对不同物种的红细胞表现出相似的溶血谱。它们对HeLa细胞和人成纤维细胞也具有细胞毒性，并在兔身上引起皮肤坏死。
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本文引用的文献
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