Mito M, Ebata H, Kusano M, Onishi T, Saito T, Sakamoto S
Transplantation. 1979 Dec;28(6):499-505. doi: 10.1097/00007890-197912000-00013.
Hepatocytes isolated by the collagenase digestive method were transplanted into the spleens of syngeneic rats. Morphology and function of the hepatocytes in the spleen were investigated for 12 to 17 months after transplantation. The transplanted hepatocytes proliferated and reconfigured in the spleen without direct perfusion of portal venous blood and with the presence of an intact host liver. Fourteen to 17 months after transplantation, the hepatocytes which had formed a demarcated nodule occupied approximately 40% of the area of the splenic parenchyma without undifferentiation on microscopic examination. However, the weight of the hepatized spleen did not increase beyond the weight of a normal spleen and the weight of the host liver that had normal morphology also did not differ from a normal liver. Light and electron microscopic studies demonstrated differentiated cord structure and normal architecture for each heptocyte. Furthermore, the hepatized spleen synthesized albumin and glycogen as demonstrated by immunofluorescence and histochemical studies. Ammonia tolerance and indocyanine green clearance tests revealed functioning hepatocytes in the spleen proper. These results indicate that our experimental model lends itself well to investigations in cell growth mechanism and that hepatocellular transplantation has potential clinical application to compensate for impaired hepatic function.
采用胶原酶消化法分离的肝细胞被移植到同基因大鼠的脾脏中。在移植后12至17个月对脾脏中肝细胞的形态和功能进行了研究。移植的肝细胞在脾脏中增殖并重新构建，没有门静脉血的直接灌注，且宿主肝脏完整。移植后14至17个月，形成界限分明结节的肝细胞占据脾实质面积的约40%，显微镜检查未见分化异常。然而，肝化脾脏的重量并未超过正常脾脏的重量，形态正常的宿主肝脏的重量也与正常肝脏无异。光镜和电镜研究显示每个肝细胞具有分化的索状结构和正常结构。此外，免疫荧光和组织化学研究表明肝化脾脏合成白蛋白和糖原。氨耐受和吲哚菁绿清除试验显示脾脏中的肝细胞功能正常。这些结果表明，我们的实验模型非常适合用于细胞生长机制的研究，肝细胞移植在临床上有补偿肝功能受损的潜在应用价值。
