Viaz'mina T N
Arkh Anat Gistol Embriol. 1979 Nov;77(11):105-8.
An experimental quantitative histological investigation was performed to study lipids in cellular elements of the regenerated connective tissue. Flap wounds were cut on the back of white mice and granulation tissue dissected 1, 3, 7, 14 30 and 60 days after the operation was investigated. Lipids and cellular elements were revealed with Sudan III-IV black B and Nile blue. Lipid quantity was estimated by means of a modified scanning integrating microphotometer. The greatest amount of lipids in the cellular elements of the granulation tissue was revealed on the 3d day of the experiment, total optic density (TOD) of lipids in leucocytes was 0.83, TOD in histiocytes--0.6. On the 7th day the amount of lipids decreased, their TOD in leucocytes was 0.6, in histiocytes--0.4. By the 14th day only phospholipids were revealed, in histiocytes their TOD was 0.3. In subsequent days phospholipid contents continued decreasing and by the 30th day their TOD was 0.2. By the 60th day, when a scar was formed in the wound, in fact, there were no lipids in the cellular elements. The microphotometric investigation performed demonstrated qualitative and quantitative characteristics of lipids in the cellular elements of the granulation tissue in dynamics and their close dependence on the time of wound healing. It was also revealed that the regenerative process was accompanied by decreasing lipid amount in the cellular elements, and this phenomenon could be used as a peculiar test to prognosticate the process of wound healing.
进行了一项实验性定量组织学研究，以研究再生结缔组织细胞成分中的脂质。在白色小鼠背部切开皮瓣伤口，并在术后1天、3天、7天、14天、30天和60天对切除的肉芽组织进行研究。用苏丹III-IV、黑色B和尼罗蓝显示脂质和细胞成分。通过改良的扫描积分显微光度计估计脂质含量。实验第3天，肉芽组织细胞成分中脂质含量最高，白细胞中脂质的总光密度（TOD）为0.83，组织细胞中为0.6。第7天，脂质含量下降，白细胞中脂质的TOD为0.6，组织细胞中为0.4。到第14天，仅显示出磷脂，组织细胞中磷脂的TOD为0.3。在随后的几天里，磷脂含量持续下降，到第30天，其TOD为0.2。到第60天，伤口形成瘢痕时，细胞成分中实际上已无脂质。所进行的显微光度研究显示了肉芽组织细胞成分中脂质的定性和定量特征随时间的动态变化及其与伤口愈合时间的密切关系。还发现再生过程伴随着细胞成分中脂质含量的减少，这一现象可作为预测伤口愈合过程的一种特殊检测方法。
