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Determination of the absolute configuration of mono-saccharides in complex carbohydrates by capillary G.L.C.

作者信息

Gerwig G J, Kamerling J P, Vliegenthart J F

出版信息

Carbohydr Res. 1979 Dec;77:10-7. doi: 10.1016/s0008-6215(00)83788-x.

DOI:10.1016/s0008-6215(00)83788-x
PMID:519653
Abstract

The absolute configuration of neutral monosaccharides, 2-acetamido-2-deoxy sugars, and uronic acids can be determined by capillary g.l.c. on SE-30 after glycosidation with (-)-2-butanol and protection of the remaining polar groups. The method is illustrated by application to mixtures of the constituent sugars of the capsular polysaccharide from Klebsiella type 57, alpha 1-acid glycoprotein, mucus glycoproteins, and the methylated, acidic polysaccharide from the coccoliths of Emiliania huxleyi (Lohmann) Kamptner.

摘要

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