Wehmann R E, Rubenstein H A, Nisula B C
Endocr Res Commun. 1979;6(3):249-55. doi: 10.3109/07435807909061799.
A highly sensitive radioimmunoassay procedures for the measurement of serum hTSH is described which permits delineation of the entire range of values in normal subjects (0.5-4.5 microU/ml). The procedure involves the concentration of hTSH from serum by adsorption to concanavalin A covalently bound to 4B Sepharose, Lactoperoxidase iodination of hTSH, and disequilibrium assay conditions. This method utilizes commonly available radioimmunoassay materials and is convenient to perform. Our results with this assay show that patients with thyrotoxicosis of a variety of etiologies have serum hTSH levels suppressed well below the normal range.
本文描述了一种用于测量血清人促甲状腺激素（hTSH）的高灵敏度放射免疫分析方法，该方法可描绘出正常受试者的整个数值范围（0.5 - 4.5微国际单位/毫升）。该方法包括通过吸附到共价结合于4B琼脂糖的伴刀豆球蛋白A从血清中浓缩hTSH、hTSH的乳过氧化物酶碘化以及非平衡分析条件。此方法使用常见的放射免疫分析材料，操作方便。我们使用该分析方法的结果表明，各种病因的甲状腺毒症患者血清hTSH水平被抑制至远低于正常范围。
