Dean R T, Hylton W, Allison A C
Exp Cell Biol. 1979;47(6):454-62. doi: 10.1159/000162963.
Cultured mouse peritoneal macrophages were shown to secrete the lysosomal enzyme N-acetyl-glucosaminidase (N-ac-Glu) in response to IgG-Sepharose and some other non-endocytosable particles without substantial release of the cytoplasmic enzyme, lactate dehydrogenase. The interaction with IgG-Sepharose was studied in some detail, and was shown to be time- and dose-dependent, and to leave the cells viable. Ovalbumin and glycine insolubilised on Sepharose did not induce secretion. By means of several control experiments, it was demonstrated that the IgG-Sepharose exerted its effects directly on the plasma membrane. Thus, normal macrophages can secrete in response to certain agents which act solely on the plasma membrane. This mechanism of induction of secretion is probably quite distinct from those previously established, which involve secretion during phagocytosis, during intracellular storage of phagocytosed materials or during pharmacological intervention by cytochalasin B.
已证明，培养的小鼠腹膜巨噬细胞在接触IgG-琼脂糖和其他一些不可内吞的颗粒时会分泌溶酶体酶N-乙酰葡糖胺酶（N-ac-Glu），而细胞质酶乳酸脱氢酶不会大量释放。对与IgG-琼脂糖的相互作用进行了较为详细的研究，结果表明这种相互作用具有时间和剂量依赖性，且细胞仍保持活力。固定在琼脂糖上的卵清蛋白和甘氨酸不会诱导分泌。通过多个对照实验证明，IgG-琼脂糖直接作用于质膜。因此，正常巨噬细胞可对仅作用于质膜的某些因子作出分泌反应。这种分泌诱导机制可能与先前确立的机制截然不同，先前的机制涉及吞噬作用期间、吞噬物质的细胞内储存期间或细胞松弛素B的药理干预期间的分泌。
