Panula P, Rechardt L
Histochemistry. 1979 Nov;64(1):35-50. doi: 10.1007/BF00493353.
The development of acetylcholinesterase (AChE) and non-specific cholinesterase (NsChE) activity was studied in the rat neostriatum by the light and electron microscope using three thiocholine methods. The AChE activity was first demonstrable only in the lateral parts of the nucleus, and during the early postnatal development the most intense activity was in the cell bodies, whilst the typical intense staining of the neuropil of adult animals was seen in two-week-old rats. Two types of AChE-containing cells were observed in the neostriatum of rats younger than two weeks and in cultures of newborn rat neostriatal cells. The neuropil of the cultures showed weak activity in the membranes of thin preterminal processes. In the neuropil of old rats, NsChE activity was present in the membranes of nerve cell processes. The capillary endothelial cells of newborn rats contained both AChE and NsChE. During subsequent development, the AChE activity disappeared, whilst for NsChE no change was seen in the distribution of activity seen in newborn or young adult rats less than three months old.
采用三种硫代胆碱方法，通过光学显微镜和电子显微镜研究了大鼠新纹状体中乙酰胆碱酯酶（AChE）和非特异性胆碱酯酶（NsChE）活性的发育情况。AChE活性最初仅在细胞核的外侧部分可见，在出生后早期发育过程中，最强烈的活性存在于细胞体中，而成年动物神经纤维网典型的强烈染色在两周大的大鼠中可见。在两周龄以下大鼠的新纹状体和新生大鼠新纹状体细胞培养物中观察到两种含AChE的细胞。培养物的神经纤维网在细的终末前突起的膜中显示出微弱的活性。在老年大鼠的神经纤维网中，NsChE活性存在于神经细胞突起的膜中。新生大鼠的毛细血管内皮细胞同时含有AChE和NsChE。在随后的发育过程中，AChE活性消失，而对于NsChE，在新生或小于三个月龄的年轻成年大鼠中观察到的活性分布没有变化。
