Waris T, Löfstedt T, Husa T, Partanen S, von Smitten K
Scand J Plast Reconstr Surg. 1984;18(2):181-5. doi: 10.3109/02844318409052835.
The regeneration of cholinesterase positive nerves into segments of the supradiaphragmatic inferior vena cava transplanted from one rat into the abdominal aorta of another rat of the same inbred strain and sex were studied, using a histochemical thiocholine method for the demonstration of cholinesterase. Iso-OMPA and BW-284 C 51 were used as inhibitors for nonspecific cholinesterase (nsChE) and acetylcholinesterase (AChE), respectively. A total of 12 grafts were examined 3, 5, 8, 16, 27 and 32 weeks after transplantation. One single regenerating nsChE reactive nerve was seen near the suture line in a 3-week-old graft. Some solitary regenerating nerves were regularly seen in the grafts at 5 weeks. Eight weeks after grafting some solitary sparsely distributed nerves were found in the outer layers of the graft. In the 16-, 27-, and 32-week-old grafts, nsChE reactive nerves and small nerve bundles were running either solitary or forming nerve plexa in addition to nerves in the vicinity of vasa vasorum. These regenerated nsChE reactive nerves are probably vasomotor in nature. The AChE reaction showed a clear activity in the intimal layer of the grafted veins. No AChE reactive nerves were seen in the 3-, 5-, and 8-week-old grafts. Only occasional AChE positive nerves were observed 16, 28 and 32 weeks after transplantation. These nerves are probably sympathetic cholinergic nerves.
采用组织化学硫代胆碱法来显示胆碱酯酶，研究了同一近交系且性别相同的大鼠体内，膈上腔静脉段移植到另一大鼠腹主动脉后胆碱酯酶阳性神经的再生情况。异稻瘟净和BW - 284 C 51分别用作非特异性胆碱酯酶（nsChE）和乙酰胆碱酯酶（AChE）的抑制剂。在移植后3、5、8、16、27和32周共检查了12个移植物。在一个3周龄的移植物中，在缝合线附近可见一条单一的再生nsChE反应性神经。在5周龄的移植物中经常可见一些孤立的再生神经。移植8周后，在移植物外层发现一些孤立的、分布稀疏的神经。在16周、27周和32周龄的移植物中，nsChE反应性神经和小神经束单独存在或形成神经丛，此外在血管周围还有神经。这些再生的nsChE反应性神经可能本质上是血管运动神经。AChE反应在移植静脉的内膜层显示出明显活性。在3周、5周和8周龄的移植物中未见AChE反应性神经。移植16周、28周和32周后仅偶尔观察到AChE阳性神经。这些神经可能是交感胆碱能神经。
