Husson A, Bouazza M, Buquet C, Vaillant R
In Vitro. 1984 Apr;20(4):314-20. doi: 10.1007/BF02618594.
Fetal rat hepatocytes were isolated and cultured in primary culture to investigate activity changes of arginase under defined conditions. In hormone-free medium, cultured cells maintained the enzyme activity at levels equal to that of freshly isolated cells for at least 4 d. Arginase activity could be induced by dexamethasone in hepatocytes isolated from 16.5-d-old fetuses although cells were competent to respond to glucagon only at the stage of 18.5 d. The combination of the two hormones induced greater levels of arginase activity than the individual compounds. These findings indicate that glucocorticoid and glucagon receptors appear early and sequentially before birth and reveal that cultured fetal hepatocytes provide a suitable system for the investigation of the role of hormones in the initiation of enzyme synthesis.
分离胎鼠肝细胞并进行原代培养，以研究特定条件下精氨酸酶的活性变化。在无激素培养基中，培养的细胞在至少4天内将酶活性维持在与新鲜分离细胞相当的水平。地塞米松可诱导从16.5日龄胎儿分离的肝细胞中的精氨酸酶活性，尽管细胞仅在18.5日龄阶段才有能力对胰高血糖素作出反应。两种激素联合诱导的精氨酸酶活性水平高于单独使用这两种化合物时。这些发现表明，糖皮质激素和胰高血糖素受体在出生前很早就依次出现，并揭示培养的胎儿肝细胞为研究激素在酶合成起始中的作用提供了一个合适的系统。
