来自人类肿瘤细胞系的双微体的超微结构
Ultrastructure of double minutes from a human tumor cell line.
作者信息
Barker P E, Stubblefield E
出版信息
J Cell Biol. 1979 Dec;83(3):663-6. doi: 10.1083/jcb.83.3.663.
Abstract
Double minutes (dm) have been isolated from human tumor cells by zonal centrifugation and by differential pelleting of chromosome suspsension. These methods allowed collection of dm in sufficient quantity and purity for visualization with electron microscopy. Ultrastructurally, the chromatin fibers in dm resemble thrance fragments was found. When the two isolation protocols were compared, differential pelleting was shown to increase purity twofold to 85% dm by mass. The differential pelleting procedure enables easy collection of dm in sufficient quantity and purity for chemical analysis.
摘要
通过区带离心和染色体悬液的差速沉淀，已从人肿瘤细胞中分离出双微体（dm）。这些方法能够收集到足够数量和纯度的dm，以便用电子显微镜观察。在超微结构上，发现dm中的染色质纤维类似于染色体片段。当比较这两种分离方案时，差速沉淀显示可将纯度提高两倍，达到质量分数85%的dm。差速沉淀法能够轻松收集到足够数量和纯度的dm用于化学分析。
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