Cook G M, Zalik S E, Milos N, Scott V
J Cell Sci. 1979 Aug;38:293-304. doi: 10.1242/jcs.38.1.293.
Extracts obtained from chick embryos at the pre-gastrula and gastrula stages are able to agglutinate trypsinized rabbit erythrocytes fixed with glutaraldehyde. Agglutination is inhibited by saccharides sharing a beta-D-galactopyranoside configuration. Agglutinin activity is also inhibited by desialysed fetuin which bears terminal galactose residues but not by native fetuin, desialysed agalacto-fetuin, bovine submaxillary mucin and desialysed bovine submaxillary mucin. Lectin activity is present in extracts obtained from the embryonic area of the blastoderms as well as in extracts from extra-embryonic endoderm and ectoderm. In extracts subjected to gel filtration on ECD Sepharose, lectin activity eluted between ovalbumim (mol. wt 45 000) and chymotrypsinogen (mol. wt 25 000). Under some experimental conditions, thiodigalactoside, the most potent inhibitor of lectin activity, inhibited the aggregation of cells of the extra-embryonic endoderm of the primitive chick embryo.
从原肠胚前期和原肠胚期的鸡胚中提取的物质能够凝集经胰蛋白酶处理并用戊二醛固定的兔红细胞。具有β-D-吡喃半乳糖苷构型的糖类可抑制凝集作用。凝集素活性也受到带有末端半乳糖残基的去唾液酸胎球蛋白的抑制，但未被天然胎球蛋白、去唾液酸无半乳糖胎球蛋白、牛颌下黏液素和去唾液酸牛颌下黏液素抑制。凝集素活性存在于从胚盘的胚胎区域提取的物质中，也存在于从胚外内胚层和外胚层提取的物质中。在经ECD琼脂糖凝胶过滤的提取物中，凝集素活性在卵清蛋白（分子量45000）和胰凝乳蛋白酶原（分子量25000）之间洗脱。在某些实验条件下，凝集素活性最有效的抑制剂硫代二半乳糖苷可抑制原始鸡胚胚外内胚层细胞的聚集。
