Panet A, Haseltine W A, Baltimore D, Peters G, Harada F, Dahlberg J E
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2535-9. doi: 10.1073/pnas.72.7.2535.
The ability of tryptophan tRNA (tRNATrp) to initiate reverse transcription of the 70S RNA of avian RNA tumor viruses suggested that the reverse transcriptase (RNA-dependent DNA polymerase; deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase; EC 2.7.7.7) might have a specific binding site for the tRNA. A complex of tRNATrp and the avian myeloblastosis virus reverse transcriptase has been demonstrated using chromatography on Sephadex G-100 columns. Of all the chicken tRNAs, only tRNATrp and a tRNA4Met bind to the enzyme with high enough affinity to be selected from a mixture of the chicken cell tRNAs. The ability of tRNATrp to change the sedimentation rate of the enzyme indicates that tRNATrp is not binding to a contaminant in the enzyme preparation. Treatment of the enzyme with monospecific antibody to reverse transcriptase prevented binding of tRNA as well as inhibited the DNA polymerase activity of the enzyme. The ability of reverse transcriptase to utilize tRNATrp aa a primer for DNA synthesis, therefore, appears to involve a highly specific site on the enzyme.
色氨酸转运RNA（tRNATrp）引发禽RNA肿瘤病毒70S RNA逆转录的能力表明，逆转录酶（RNA依赖性DNA聚合酶；脱氧核苷三磷酸：DNA脱氧核苷酸基转移酶；EC 2.7.7.7）可能有一个tRNA的特异性结合位点。使用Sephadex G - 100柱层析已证明tRNATrp与禽成髓细胞瘤病毒逆转录酶形成了复合物。在所有鸡的转运RNA中，只有tRNATrp和一种tRNA4Met以足够高的亲和力与该酶结合，从而能从鸡细胞转运RNA混合物中被筛选出来。tRNATrp改变该酶沉降速率的能力表明tRNATrp并非与酶制剂中的污染物结合。用逆转录酶的单特异性抗体处理该酶可阻止tRNA的结合，并抑制该酶的DNA聚合酶活性。因此，逆转录酶利用tRNATrp作为DNA合成引物的能力似乎涉及该酶上一个高度特异性的位点。
