Waller H, Friess E, Kiefer J
Radiat Environ Biophys. 1981;19(4):259-64. doi: 10.1007/BF01324091.
Denatured calf-thymus DNA was X-irradiated, coupled to methylated bovine serum albumin and injected into rabbits to study the possible formation of specific antibodies. The serological activity was tested by a modified Farr-test, a micro complement binding reaction and by caesium chloride isopycnic ultracentrifugation. It was found with all assays that the immunological reaction was mainly due to unspecific DNA-binding and only a very small amount to radiation products. It seems, therefore, that this approach is not suitable for the analytical investigation of DNA damage produced by ionizing radiation.
将变性的小牛胸腺DNA进行X射线照射，与甲基化牛血清白蛋白偶联，然后注射到兔子体内，以研究特异性抗体的可能形成。通过改良的Farr试验、微量补体结合反应和氯化铯等密度超速离心法检测血清学活性。所有检测均发现，免疫反应主要归因于非特异性DNA结合，只有极少量归因于辐射产物。因此，这种方法似乎不适用于对电离辐射产生的DNA损伤进行分析研究。
