Heymer B, Bernstein D, Schleifer K H, Krause R M
Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):168-78.
Staphylococcus epidermidis peptidoglycans solubilized by sonication or lysozyme digestion, and synthetic peptidoglycan analogs such as HSA-carboxymethyl-Gly-L-Ala-L-Ala-D-Ala-D-Ala (HSA-pentapeptide) or L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala (pentapeptide) have been labeled with 125I and tested for their applicability in the radioactive antigen binding assay. Use of radioiodinated Staph. epidermidis peptidoglycans was found to be considerably impeded by the presence of at least 2 different antigenic sites on such molecules, the pentapeptide and the glycan determinant. Application of labeled HSA-pentapeptide was limited by the necessity to use PEG for precipitation of Ag-Ab-complexes and by short linear potions of binding curves. However, the synthetic pentapeptide hapten, radioiodinated by the active ester method of BOLTON and HUNTER, proved to be a most useful regent for the selective measurement of pentapeptide antibody. Inhibition studies indicated that the immunological specificity of the labeled hapten was retained. Pentapeptide binding curves were linear from 15-500 g/ml of antibody. Generally, there was good agreement between pentapeptide antibody concentrations measured by radioimmunoassay and quantitative precipitation.
通过超声处理或溶菌酶消化溶解的表皮葡萄球菌肽聚糖，以及合成肽聚糖类似物，如HSA-羧甲基-Gly-L-Ala-L-Ala-D-Ala-D-Ala（HSA-五肽）或L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala（五肽），已用125I标记，并测试了它们在放射性抗原结合试验中的适用性。发现使用放射性碘化的表皮葡萄球菌肽聚糖会受到此类分子上至少2个不同抗原位点（五肽和聚糖决定簇）的显著阻碍。标记的HSA-五肽的应用受到使用聚乙二醇沉淀Ag-Ab复合物的必要性以及结合曲线线性部分较短的限制。然而，通过BOLTON和HUNTER的活性酯法进行放射性碘化的合成五肽半抗原，被证明是选择性测量五肽抗体的最有用试剂。抑制研究表明，标记半抗原的免疫特异性得以保留。五肽结合曲线在抗体浓度为15-500μg/ml时呈线性。一般来说，通过放射免疫测定法测量的五肽抗体浓度与定量沉淀法之间具有良好的一致性。
