Heymer B, Schleifer K H, Read S, Zabriskie J B, Krause R M
J Immunol. 1976 Jul;117(1):23-6.
A radioimmunoassay has been developed for the measurement of antibodies to peptidoglycan in human sera including patients with rheumatic fever and juvenile rheumatoid arthritis. The assay is based on the percentage of binding of the hapten 125I-L-Ala-gamma-d-Glu-L-Lys-D-Ala-D-Ala, the major peptide determinant of peptidoglycan. Because of differences in the avidity of the antibodies in different sera, the amount of antibody was expressed as pentapeptide hapten-binding capacity (pentapeptide-HBC in ng/ml of serum). Fourteen out of 105 normal blood donors had a pentapeptide-HBC value greater than or equal to 75 ng/ml serum. Values in healthy children 5 to 18 years of age were less than or equal to 50 ng/ml. Sixty-eight percent of the individuals with rheumatic fever had values greater than or equal to 75 ng/ml, an indication that streptococcal infections can stimulate an immune response to peptidoglycan. Thirty-five percent of the patients with juvenile rheumatoid arthritis had values greater than or equal to 75 ng/ml. Such a finding points to a possible association between bacterial infections and juvenile rheumatoid arthritis.
已开发出一种放射免疫分析法，用于测量人血清中针对肽聚糖的抗体，包括风湿热患者和青少年类风湿性关节炎患者的血清。该分析基于半抗原125I-L-丙氨酸-γ-D-谷氨酸-L-赖氨酸-D-丙氨酸-D-丙氨酸（肽聚糖的主要肽决定簇）的结合百分比。由于不同血清中抗体亲和力存在差异，抗体量以五肽半抗原结合能力（血清中五肽-HBC，单位为ng/ml）表示。105名正常献血者中有14人的五肽-HBC值大于或等于75 ng/ml血清。5至18岁健康儿童的值小于或等于50 ng/ml。68%的风湿热患者的值大于或等于75 ng/ml，这表明链球菌感染可刺激对肽聚糖的免疫反应。35%的青少年类风湿性关节炎患者的值大于或等于75 ng/ml。这一发现表明细菌感染与青少年类风湿性关节炎之间可能存在关联。
