McKinney R M, Spillane J T
Ann N Y Acad Sci. 1975 Jun 30;254:55-64. doi: 10.1111/j.1749-6632.1975.tb29155.x.
A survey of commercially available TMRI and RBI products revealed that many are of unsatisfactory quality. Optimum dye/IgG ratios were obtained in conjugates with TMRI of about 65% purity by labeling them with reaction mixture ratios of 10 and 20 mug dye/mg IgG. Optimum labeling with RBI of comparable purity required reaction mixture ratios of at least 20 mug dye/mg IgG. Although specific storage stability studies of TMRI or RBI products were not performed, one TMRI product underwent appreciable degradation during a 2-year period of storage in a desiccator over Drierite. The importance of evaluating the protein labeling capacity of TMRI or RBI products before making immune conjugate preparations is evident.
对市售的噻吗心安放射性碘（TMRI）和放射性铼（RBI）产品的一项调查显示，许多产品质量不尽人意。通过用10和20微克染料/毫克免疫球蛋白G（IgG）的反应混合物比例进行标记，在纯度约为65%的TMRI缀合物中获得了最佳染料/I IgG比例。具有相当纯度的RBI的最佳标记需要至少20微克染料/毫克IgG的反应混合物比例。尽管未对TMRI或RBI产品进行特定的储存稳定性研究，但一种TMRI产品在干燥器中置于干燥剂上储存两年期间发生了明显降解。在制备免疫缀合物之前评估TMRI或RBI产品的蛋白质标记能力的重要性是显而易见的。
