Infertility in the turkey. I. Effects of anti-sperm immune globulins on fertilizing ability of turkey spermatozoa.

Two experiments were conducted to study the effects of globulins, isolated from sera of turkey hens immunized against semen, on the fertilizing ability of spermatozoa and the hatchability of eggs. Sperm were incubated in sera from hens immunized against semen and in sera from non-immunized hens as well as in globulin solutions isolated from these various sera. In neither experiment did sera from control hens or gamma globulins isolated from their sera affect the fertilizing ability of sperm. In contrast, all sera from immunized hens drastically reduced the fertilizing ability of sperm. In Experiment 1, .75% gamma globulin solutions from two hens did not depress fertility but a 1.5% solution from one of the immunized hens significantly (P less than .05) reduced it. In this experiment none of the treatments had a significant effect on hatchability. Antisemen globulin solutions of 2.25 and 4.50% from 12 hens significantly reduced the fertilizing ability of sperm in dose related fashion. The higher of the two levels suppressed it to the level of whole sera. Hatchability of fertile eggs was significantly reduced by the anti-semen sera and globulins isolated from it in the second experiment.