Burke W H, Yu W C
Poult Sci. 1979 Sep;58(5):1367-71. doi: 10.3382/ps.0581367.
Two experiments were conducted to study the effects of globulins, isolated from sera of turkey hens immunized against semen, on the fertilizing ability of spermatozoa and the hatchability of eggs. Sperm were incubated in sera from hens immunized against semen and in sera from non-immunized hens as well as in globulin solutions isolated from these various sera. In neither experiment did sera from control hens or gamma globulins isolated from their sera affect the fertilizing ability of sperm. In contrast, all sera from immunized hens drastically reduced the fertilizing ability of sperm. In Experiment 1, .75% gamma globulin solutions from two hens did not depress fertility but a 1.5% solution from one of the immunized hens significantly (P less than .05) reduced it. In this experiment none of the treatments had a significant effect on hatchability. Antisemen globulin solutions of 2.25 and 4.50% from 12 hens significantly reduced the fertilizing ability of sperm in dose related fashion. The higher of the two levels suppressed it to the level of whole sera. Hatchability of fertile eggs was significantly reduced by the anti-semen sera and globulins isolated from it in the second experiment.
进行了两项实验，以研究从免疫精液的母火鸡血清中分离出的球蛋白对精子受精能力和蛋孵化率的影响。精子分别在免疫精液的母鸡血清、未免疫母鸡血清以及从这些不同血清中分离出的球蛋白溶液中孵育。在两项实验中，对照母鸡的血清或从其血清中分离出的γ球蛋白均未影响精子的受精能力。相比之下，所有免疫母鸡的血清都大幅降低了精子的受精能力。在实验1中，来自两只母鸡的0.75%γ球蛋白溶液未降低受精率，但来自其中一只免疫母鸡的1.5%溶液显著（P小于0.05）降低了受精率。在该实验中，所有处理对孵化率均无显著影响。来自12只母鸡的2.25%和4.50%抗精液球蛋白溶液以剂量相关方式显著降低了精子的受精能力。两个水平中较高的那个将其抑制到了全血清的水平。在第二个实验中，抗精液血清及其分离出的球蛋白显著降低了受精卵的孵化率。
