己糖激酶同工酶抗原位点和硫醇抑制剂敏感位点的鉴别
Discrimination of antigenic site and thiol-inhibitor-sensitive site of hexokinase isoenzymes.
作者信息
Ouchi M, Ishibashi S
出版信息
Biochem J. 1975 Aug;149(2):481-3. doi: 10.1042/bj1490481.
Abstract
Rabbit antiserum was prepared against hexokinase isoenzyme type I which was purified from rat brain mitochondria. The antiserum inhibited the activity of the mitochondrial hexokinase type I as well as that of the cytosolic type I enzyme prepared from rat brain, kidney and spleen. It did not, however, inhibit the activity of type II hexokinase from muscle and spleen or that of the type III enzyme from spleen. The results suggest that all hexokinase type I isoenzymes may have a common antigenic site irrespective of their sources, though their responses to a thiol inhibitor are different.
摘要
用从大鼠脑线粒体中纯化得到的己糖激酶同工酶I制备兔抗血清。该抗血清抑制线粒体己糖激酶I的活性，以及从大鼠脑、肾和脾制备的胞质型I酶的活性。然而，它不抑制来自肌肉和脾的II型己糖激酶的活性，也不抑制来自脾的III型酶的活性。结果表明，所有的I型己糖激酶同工酶可能都有一个共同的抗原位点，而不论其来源如何，尽管它们对巯基抑制剂的反应不同。
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