大鼠肝脏中腺苷5'-三磷酸-D-葡萄糖6-磷酸转移酶的进一步性质及可能的作用机制
Further properties and possibel mechanism of action of adenosine 5'-triphosphate-D-glucose 6-phosphotransferase from rat liver.
作者信息
Parry M J, Walker D G
出版信息
Biochem J. 1967 Nov;105(2):473-82. doi: 10.1042/bj1050473.
Abstract
- Magnesium ions are the most effective bivalent ions in the glucokinase reaction. 2. The molecular weight of rat hepatic glucokinase is 48000-49000 as assessed by gel filtration on Sephadex G-100. 3. Anomalous kinetic behaviour at low glucose concentrations appears to be due to the formation during the purification procedure of fragments possessing modified catalytic properties, but is unlikely to be of physiological significance. 4. Extension of previous studies (Parry & Walker, 1966) suggests that glucokinase catalyses a reaction of the random Bi Bi type similar to that of yeast hexokinase. 5. The inhibitory effects of various thiol reagents suggest that a thiol group may be involved at or near the binding site of the acceptor molecule.
摘要
- 镁离子是葡萄糖激酶反应中最有效的二价离子。2. 通过在葡聚糖凝胶G - 100上进行凝胶过滤评估，大鼠肝脏葡萄糖激酶的分子量为48000 - 49000。3. 低葡萄糖浓度下的异常动力学行为似乎是由于在纯化过程中形成了具有修饰催化特性的片段，但不太可能具有生理意义。4. 先前研究（Parry & Walker，1966）的扩展表明，葡萄糖激酶催化的反应类似于酵母己糖激酶的随机双底物双产物类型反应。5. 各种巯基试剂的抑制作用表明，巯基可能在受体分子的结合位点处或附近起作用。
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A study of the 'reactive' sulphydryl groups of adenosine 5'-triphosphate-creatine phosphotransferase.三磷酸腺苷-磷酸肌酸磷酸转移酶“反应性”巯基的研究
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Kinetic studies of the brain hexokinase reaction.大脑己糖激酶反应的动力学研究。
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