Vince J D, McManus T J, Ferguson-Smith M A, Ratcliffe J G
Br J Obstet Gynaecol. 1975 Sep;82(9):718-27. doi: 10.1111/j.1471-0528.1975.tb00712.x.
A simple, reliable, semi-automated and inexpensive radioimmunoassay for serum alphafetoprotein (AFP) is described which fulfils the requirements for a screening test for open neural tube defects. The normal range for serum AFP throughout pregnancy has been determined in 1534 samples. In a retrospective study of 15 samples of maternal serum taken from nine pregnant women carrying a fetus affected with a neural tube defect, the serum AFP level was found to be above the 95th centile in four of the five cases of open defect sampled between 15 and 20 weeeks gestation. Preliminary results of a prospective study are reported in which two cases of nencephaly were detected among 671 unselected pregnancies sampled between 15 and 20 weeks gestation.
本文描述了一种简单、可靠、半自动且廉价的血清甲胎蛋白（AFP）放射免疫测定法，该方法满足开放性神经管缺陷筛查试验的要求。已对1534份样本测定了整个孕期血清AFP的正常范围。在一项回顾性研究中，从9名怀有神经管缺陷胎儿的孕妇采集了15份母血样本，发现在妊娠15至20周期间采样的5例开放性神经管缺陷病例中，有4例的血清AFP水平高于第95百分位数。报告了一项前瞻性研究的初步结果，在妊娠15至20周期间采样的671例未经选择的孕妇中，检测出2例无脑儿。
