Naroditsky B S, Zavizion B A, Karamov E V, Tikhonenko T I
Nucleic Acids Res. 1978 Mar;5(3):999-1011. doi: 10.1093/nar/5.3.999.
The effect of the restricting endonucleases R.EcoRI, R.BamI and R.SalI on the genome of type 7 simian adenovirus (SA-7) has been studied. Since the DNA has only one site of R.EcoRI recognition the enzyme cleaves SA-7 DNA into two fragments with the molecular weights 12.0 and 10.0 . 10(6). The restrictase R.BamI cleaves the SA-7 DNA at six sites producing 7 fragments with the molecular weights 6.6, 5.9, 3.8, 2.7, 1.3, 0.7 and 0.6 . 10(6). R.SalI cleavage yields 6 fragments with the molecular weights 8.1, 5.5, 4.3, 2.45, 1.2 and 0.6 . 10(6). The R.BamI and R.SalI fragments are arranged in the orders E-A-D-F-C-G-B and A-B-D-F-E-C, respectively. The only R.EcoRI recognition site is localized in the C fragment produced by R.BamI and in the B fragment produced by R.SalI.
研究了限制性内切酶R.EcoRI、R.BamI和R.SalI对7型猿猴腺病毒（SA-7）基因组的影响。由于该DNA只有一个R.EcoRI识别位点，该酶将SA-7 DNA切割成两个分子量分别为12.0和10.0×10⁶的片段。限制性内切酶R.BamI在六个位点切割SA-7 DNA，产生七个分子量分别为6.6、5.9、3.8、2.7、1.3、0.7和0.6×10⁶的片段。R.SalI切割产生六个分子量分别为8.1、5.5、4.3、2.45、1.2和0.6×10⁶的片段。R.BamI和R.SalI片段分别按E-A-D-F-C-G-B和A-B-D-F-E-C的顺序排列。唯一的R.EcoRI识别位点位于R.BamI产生的C片段和R.SalI产生的B片段中。
