Ziska P, Franz H
Acta Biol Med Ger. 1979;38(4):697-700.
The D-galactose specific lectin from Viscum album and the D-mannose specific lectin from Vicia faba react with serum proteins which contain the corresponding carboyhydrate moieties. By affinity chromatography of human serum using the combination of insolubilized lectins coupled to Sepharose it is possible to fractionate serum proteins in 3 groups: 1. proteins which react with both of the lectins; 2. proteins which react only with one of the lectins; 3. proteins which do not display any affinity for one of the two lectins.
来自欧洲槲寄生的D-半乳糖特异性凝集素和来自蚕豆的D-甘露糖特异性凝集素与含有相应碳水化合物部分的血清蛋白发生反应。通过使用偶联到琼脂糖上的不溶性凝集素组合对人血清进行亲和层析，可以将血清蛋白分为3组：1. 与两种凝集素都发生反应的蛋白；2. 仅与其中一种凝集素发生反应的蛋白；3. 对两种凝集素中的任何一种都没有亲和力的蛋白。
