Dor I, Namba M, Sato J
Gan. 1975 Aug;66(4):385-92.
Two cell lines of human hepatoma, HLE and HLF lines, were established in vitro from the hepatocellular carcinoma of a 68-year-old patient. One clone (HLEC) was obtained from a single HLE cell. The cells of HLE and HLEC were epithelial-like and both of these cells demonstrated glycogen granules in the cytoplasm when stained with periodic acid and Schiff reagent. Although HLF cells resembled fibroblasts in morphology, they appear to have originated from hepatoma cells, judging from epithelial characteristics in aggregates reconstituted by rotation culture and heterotransplantability. HLE cells produced alpha-fetoprotein until day 187 of culture, but HLF cells did not produce alpa-fetoprotein at any period examined. Chromosome number of both cell lines was distributed near the triploid range. HLF cells were transplantable into the cheek pouch of adult hamsters treated with cortisone acetate, but not HLE cells.
从一名68岁患者的肝细胞癌中体外建立了两株人肝癌细胞系，即HLE和HLF细胞系。从单个HLE细胞获得了一个克隆（HLEC）。HLE和HLEC细胞呈上皮样，用高碘酸和席夫试剂染色时，这两种细胞的细胞质中均显示出糖原颗粒。尽管HLF细胞在形态上类似于成纤维细胞，但从旋转培养重建聚集体中的上皮特征和异种移植性判断，它们似乎起源于肝癌细胞。HLE细胞在培养的第187天之前产生甲胎蛋白，但HLF细胞在任何检测时期均未产生甲胎蛋白。两株细胞系的染色体数分布在近三倍体范围内。HLF细胞可移植到用醋酸可的松处理的成年仓鼠的颊囊中，但HLE细胞不能。
