Alpha-fetoprotein and albumin produced by subclonal cell population of the ascites hepatoma AH-66 in vitro.

Subclonal cell populations were prepared from a clonal line of the rat ascites hepatoma AH-66 in vitro, using the agar plate culture method. alpha-Fetoprotein and albumin concentrations in culture media of these subclones were determined by 125I-radioimmunoassay and single radial immunodiffusion method, respectively. Results demonstrated that the AH-66 clone was a complex of cells with varying producibility of alpha-fetoprotein and albumin. All the subclones showed varied and distinct production of alpha-fetoprotein, but not all the subclones produced detectable levels of albumin.