Isaka H, Umehara S, Yoshii H, Tsukada Y, Hirai H
Gan. 1976 Feb;67(1):131-5.
Subclonal cell populations were prepared from a clonal line of the rat ascites hepatoma AH-66 in vitro, using the agar plate culture method. alpha-Fetoprotein and albumin concentrations in culture media of these subclones were determined by 125I-radioimmunoassay and single radial immunodiffusion method, respectively. Results demonstrated that the AH-66 clone was a complex of cells with varying producibility of alpha-fetoprotein and albumin. All the subclones showed varied and distinct production of alpha-fetoprotein, but not all the subclones produced detectable levels of albumin.
采用琼脂平板培养法，在体外从大鼠腹水肝癌AH-66的克隆系中制备亚克隆细胞群体。分别通过125I放射免疫测定法和单向免疫扩散法测定这些亚克隆的培养基中甲胎蛋白和白蛋白的浓度。结果表明，AH-66克隆是一个甲胎蛋白和白蛋白产生能力各异的细胞复合体。所有亚克隆甲胎蛋白的产生情况各不相同且有明显差异，但并非所有亚克隆都能产生可检测水平的白蛋白。
