Lun A, Stigge V, Ziemer S, Strauss D, Raderecht H J
Folia Haematol Int Mag Klin Morphol Blutforsch. 1975;102(3):335-45.
If the pH value in the ACD or in the ACD-AG storage solution is enhanced, the glucose in the autoclaving with undergo a caramelizing process. For this reason glucose was replaced by sorbite in the storage solutions with a pH value of 6.0 and additions of xylitol and pyruvate. The initial pH value in the blood amounted to 7.3. The content of 2.3 DPG of the erythrocytes remained fully preserved in the blood with sorbitol and additions of xylitol and pyruvate during the first 2 weeks of storage and decreased to 30% only in the third week. There were only slight amounts of 2.3 DPG in the ACD-AG blood at that time of storage. Up to the third week of storage the ATP content of erythrocytes as well as the haemoglobin level in the plasma revealed no essential differences between stored blood with sorbitol and xylitol as a substrate or glucose + xylitol respectively. The quick decrease of the ATP level to zero and the simultaneous strong increase of haemolysis in the sorbitol blood within the fourth week of storage is discussed in connection with a lowering of the NAD/NADH2 quotient. For the purpose of keeping the 2.3 DPG level of erythrocytes a storage solution with sorbite and xylitol (ASCX-AG-Pyr 10mM) seems to be well suited for a storing time of 2---3 weeks at first.
如果ACD或ACD-AG储存溶液中的pH值升高，高压灭菌过程中的葡萄糖会发生焦糖化反应。因此，在pH值为6.0且添加木糖醇和丙酮酸的储存溶液中，葡萄糖被山梨醇取代。血液的初始pH值为7.3。在储存的前两周，添加山梨醇、木糖醇和丙酮酸的血液中红细胞的2,3-DPG含量完全保持不变，仅在第三周降至30%。在储存时，ACD-AG血液中只有少量的2,3-DPG。在储存的前三周，以山梨醇和木糖醇为底物或分别以葡萄糖+木糖醇储存的血液中，红细胞的ATP含量以及血浆中的血红蛋白水平没有本质差异。储存第四周时，山梨醇血液中ATP水平迅速降至零，同时溶血现象强烈增加，这与NAD/NADH2商数降低有关。为了保持红细胞的2,3-DPG水平，含有山梨醇和木糖醇的储存溶液（ASCX-AG-Pyr 10mM）最初似乎非常适合2至3周的储存时间。
