Holthausen H S, Chacko S, Davidson E A, Holtzer H
Proc Natl Acad Sci U S A. 1969 Jul;63(3):864-70. doi: 10.1073/pnas.63.3.864.
Exposure of cultured cartilage cells to 5-bromodeoxyuridine results in a rapid loss of the ability to synthesize the chondroitin 4-sulfate-protein complex characteristic of the extracellular matrix of differentiated cartilage. UDP-glucose dehydrogenase, UDP-N-acetyl hexosamine 4-epimerase and the enzyme system responsible for catalyzing 3'-phosphoadenosine-5'-phosphosulfate synthesis all progressively decline in activity, while several other cellular processes seem unaffected. However, there are marked morphological changes which may be associated with plasma membrane components or the synthesis of the protein-polysaccharide complex.
将培养的软骨细胞暴露于5-溴脱氧尿苷会导致其迅速丧失合成分化软骨细胞外基质特有的硫酸软骨素4-硫酸盐-蛋白质复合物的能力。UDP-葡萄糖脱氢酶、UDP-N-乙酰己糖胺4-表异构酶以及负责催化3'-磷酸腺苷-5'-磷酸硫酸合成的酶系统的活性均逐渐下降，而其他几个细胞过程似乎未受影响。然而，存在明显的形态学变化，这些变化可能与质膜成分或蛋白质-多糖复合物的合成有关。
