5-溴脱氧尿苷对体外培养软骨细胞表达的影响。
Effect of 5-bromodeoxyuridine on expression of cultured chondrocytes grown in vitro.
作者信息
Holthausen H S, Chacko S, Davidson E A, Holtzer H
出版信息
Proc Natl Acad Sci U S A. 1969 Jul;63(3):864-70. doi: 10.1073/pnas.63.3.864.
Abstract
Exposure of cultured cartilage cells to 5-bromodeoxyuridine results in a rapid loss of the ability to synthesize the chondroitin 4-sulfate-protein complex characteristic of the extracellular matrix of differentiated cartilage. UDP-glucose dehydrogenase, UDP-N-acetyl hexosamine 4-epimerase and the enzyme system responsible for catalyzing 3'-phosphoadenosine-5'-phosphosulfate synthesis all progressively decline in activity, while several other cellular processes seem unaffected. However, there are marked morphological changes which may be associated with plasma membrane components or the synthesis of the protein-polysaccharide complex.
摘要
将培养的软骨细胞暴露于5-溴脱氧尿苷会导致其迅速丧失合成分化软骨细胞外基质特有的硫酸软骨素4-硫酸盐-蛋白质复合物的能力。UDP-葡萄糖脱氢酶、UDP-N-乙酰己糖胺4-表异构酶以及负责催化3'-磷酸腺苷-5'-磷酸硫酸合成的酶系统的活性均逐渐下降，而其他几个细胞过程似乎未受影响。然而，存在明显的形态学变化，这些变化可能与质膜成分或蛋白质-多糖复合物的合成有关。
相似文献
1
Effect of 5-bromodeoxyuridine on expression of cultured chondrocytes grown in vitro.5-溴脱氧尿苷对体外培养软骨细胞表达的影响。
Proc Natl Acad Sci U S A. 1969 Jul;63(3):864-70. doi: 10.1073/pnas.63.3.864.
2
Synthesis of proteochondroitin sulfate by normal, nanomelic, and 5-bromodeoxyuridine-treated chondrocytes in cell culture.正常、短肢型及经5-溴脱氧尿苷处理的软骨细胞在细胞培养中合成硫酸软骨素蛋白聚糖。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3385-8. doi: 10.1073/pnas.69.11.3385.
3
4
The synthesis of matrix components by chondrocytes in vitro in the presence of 5-bromodeoxyuridine.软骨细胞在5-溴脱氧尿苷存在的情况下于体外合成基质成分。
Cell Differ. 1973 Dec;2(5):285-98. doi: 10.1016/0045-6039(73)90033-x.
5
Suppression of chondrogenic expression in mixtures of normal chondrocytes and BUDR-altered chondrocytes grown in vitro.体外培养的正常软骨细胞与经溴脱氧尿苷（BUDR）处理的软骨细胞混合物中软骨生成表达的抑制。
Biochem Biophys Res Commun. 1969 Jan 27;34(2):183-9. doi: 10.1016/0006-291x(69)90629-9.
6
Control of chondrogenesis in limb-bud cell cultures by bromodeoxyuridine.溴脱氧尿苷对肢芽细胞培养中软骨形成的控制
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2201-5. doi: 10.1073/pnas.70.8.2201.
7
Regulation of cartilage enzymes in cultured chondrocytes and the effect of 5-bromodeoxyuridine.培养软骨细胞中软骨酶的调控及5-溴脱氧尿苷的作用
Dev Biol. 1972 Jan;27(1):20-6. doi: 10.1016/0012-1606(72)90109-1.
8
The loss of phenotypic traits by differentiated cells, V. The effect of 5-bromodeoxyuridine on cloned chondrocytes.分化细胞表型特征的丧失，V. 5-溴脱氧尿苷对克隆软骨细胞的影响。
Proc Natl Acad Sci U S A. 1968 Apr;59(4):1144-51. doi: 10.1073/pnas.59.4.1144.
9
Regulatory aspects of glycosaminoglycan biosynthesis.糖胺聚糖生物合成的调控方面。
Acta Biol Hung. 1984;35(2-4):109-21.
10
The irreversible effect of bromodeoxyuridine on chick chondrocytes.溴脱氧尿苷对鸡软骨细胞的不可逆作用。
Exp Cell Res. 1982 May;139(1):79-86. doi: 10.1016/0014-4827(82)90320-2.
引用本文的文献
1
Induction and inhibition of Friend erythroleukemia cell differentiation by pyrimidine analogs: analysis of the requirement for intracellular accumulation and incorporation into DNA.嘧啶类似物对Friend红白血病细胞分化的诱导与抑制：细胞内积累及掺入DNA的需求分析
Mol Cell Biol. 1982 Aug;2(8):1020-4. doi: 10.1128/mcb.2.8.1020-1024.1982.
2
3
The effects of 5-bromodeoxyuridine on yolk sac erythropoiesis in the chick embryo.5-溴脱氧尿苷对鸡胚卵黄囊红细胞生成的影响。
J Cell Biol. 1971 Mar;48(3):523-32. doi: 10.1083/jcb.48.3.523.
4
Erythropoietic cell cultures from chick embryos.来自鸡胚的红细胞生成细胞培养物。
J Cell Biol. 1972 Jul;54(1):98-106. doi: 10.1083/jcb.54.1.98.
5
Suppression of malignancy and differentiation in melanotic melanoma cells.黑色素瘤细胞中恶性肿瘤的抑制与分化
Proc Natl Acad Sci U S A. 1970 May;66(1):72-8. doi: 10.1073/pnas.66.1.72.
6
Lac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor.乳糖操纵子类似物：乳糖操纵子中的溴脱氧尿苷取代影响乳糖阻遏物的解离速率。
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2574-6. doi: 10.1073/pnas.69.9.2574.
7
Cytomegalovirus replication in cells pretreated with 5-iodo-2'-deoxyuridine.巨细胞病毒在经5-碘-2'-脱氧尿苷预处理的细胞中的复制。
J Virol. 1973 Jun;11(6):986-90. doi: 10.1128/JVI.11.6.986-990.1973.
8
Influence of external potassium on the synthesis and deposition of matrix components by chondrocytes in vitro.体外环境中钾离子对软骨细胞基质成分合成与沉积的影响
J Cell Biol. 1974 Dec;63(3):843-54. doi: 10.1083/jcb.63.3.843.
9
Control of chondrogenesis in limb-bud cell cultures by bromodeoxyuridine.溴脱氧尿苷对肢芽细胞培养中软骨形成的控制
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2201-5. doi: 10.1073/pnas.70.8.2201.
10
Protein-polyaccharide of chicken cartilage and chondrocyte cell cultures.鸡软骨和软骨细胞培养物的蛋白质多糖
Biochem J. 1970 Dec;120(4):689-97. doi: 10.1042/bj1200689.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.哺乳动物细胞在化学成分明确的合成培养基中的克隆生长
Proc Natl Acad Sci U S A. 1965 Feb;53(2):288-93. doi: 10.1073/pnas.53.2.288.
3
Acid-soluble nucleotide spectrum of Neurospora crassa.粗糙脉孢菌的酸溶性核苷酸谱
Arch Biochem Biophys. 1960 Feb;86:267-9. doi: 10.1016/0003-9861(60)90416-1.
4
The loss of phenotypic traits by differentiated cells. IV. Changes in polysaccharides produced by dividing chondrocytes.分化细胞表型特征的丧失。IV. 分裂软骨细胞产生的多糖变化。
Dev Biol. 1967 Sep;16(3):250-81. doi: 10.1016/0012-1606(67)90026-7.
5
Suppression of chondrogenic expression in mixtures of normal chondrocytes and BUDR-altered chondrocytes grown in vitro.体外培养的正常软骨细胞与经溴脱氧尿苷（BUDR）处理的软骨细胞混合物中软骨生成表达的抑制。
Biochem Biophys Res Commun. 1969 Jan 27;34(2):183-9. doi: 10.1016/0006-291x(69)90629-9.
6
Cellular differentiation and the aging process in cartilaginous tissues. Mucopolysaccharide synthesis in cell cultures of chondrocytes.软骨组织中的细胞分化与衰老过程。软骨细胞培养中的黏多糖合成。
J Exp Med. 1968 Dec 1;128(6):1353-62. doi: 10.1084/jem.128.6.1353.
7
Mitosis and intermediate-sized filaments in developing skeletal muscle.发育中的骨骼肌中的有丝分裂和中间丝
J Cell Biol. 1968 Sep;38(3):538-55. doi: 10.1083/jcb.38.3.538.
8
An analysis of myogenesis in vitro using fluorescein-labeled antimyosin.使用荧光素标记的抗肌球蛋白对体外肌生成进行分析。
J Histochem Cytochem. 1965 Nov-Dec;13(8):726-39. doi: 10.1177/13.8.726.
9
The loss of phenotypic traits by differentiated cells, V. The effect of 5-bromodeoxyuridine on cloned chondrocytes.分化细胞表型特征的丧失，V. 5-溴脱氧尿苷对克隆软骨细胞的影响。
Proc Natl Acad Sci U S A. 1968 Apr;59(4):1144-51. doi: 10.1073/pnas.59.4.1144.
10
Enzymatic synthesis of DNA, XXIV. Synthesis of infectious phage phi-X174 DNA.DNA的酶促合成，XXIV. 传染性噬菌体φ-X174 DNA的合成
Proc Natl Acad Sci U S A. 1967 Dec;58(6):2321-8. doi: 10.1073/pnas.58.6.2321.
Zotero 插件