乳糖操纵子类似物：乳糖操纵子中的溴脱氧尿苷取代影响乳糖阻遏物的解离速率。
Lac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor.
作者信息
Lin S Y, Riggs A D
出版信息
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2574-6. doi: 10.1073/pnas.69.9.2574.
Abstract
As measured by a decreased rate of dissociation, lac repressor binds 10-times tighter to 5-bromodeoxyuridine-substituted lac operator than it does to normal lac operator. This result is obtained both in the absence and in the presence of isopropylthiogalactoside, an inducing ligand. These data are significant with regard to the mechanism of sequence-specific protein-DNA interaction, and also suggest a possible explanation for the effects of bromodeoxyuridine on the expression of differentiated functions in eukaryotic cells.
摘要
通过解离速率降低来衡量，乳糖阻遏物与5-溴脱氧尿苷取代的乳糖操纵基因的结合比与正常乳糖操纵基因的结合紧密10倍。在不存在和存在诱导配体异丙基硫代半乳糖苷的情况下均得到该结果。这些数据对于序列特异性蛋白质-DNA相互作用的机制具有重要意义，并且还为溴脱氧尿苷对真核细胞中分化功能表达的影响提供了一种可能的解释。
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Lac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor.乳糖操纵子类似物：乳糖操纵子中的溴脱氧尿苷取代影响乳糖阻遏物的解离速率。
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2574-6. doi: 10.1073/pnas.69.9.2574.
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