毛霉中一种类胡萝卜素生成酶聚集体：来自定量互补的证据
A carotenogenic enzyme aggregate in Phycomyces: evidence from quantitive complementation.
作者信息
De la Guardia M D, Aragón C M, Murillo F J, Cerdá-Olmedo E
出版信息
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2012-5. doi: 10.1073/pnas.68.9.2012.
Abstract
Wild-type Phycomyces blakesleeanus accumulates beta-carotene, while the mutant strain C2 is unable to synthesize carotenoids, and the mutant strain C9 accumulates lycopene. Heterokaryons containing a proportion, p, of C2 nuclei and (l - p) of C9 nuclei accumulate lycopene, gamma-carotene, and beta-carotene in the relative amounts (l - p), p(l - p), and p(2), respectively, for different values of p. It is shown that these results are expected from the operation of a carotenogenic enzyme aggregate that works as an assembly line and contains two copies of a cyclase, which is defective in strain C9, as well as other enzymes.
摘要
野生型布氏毛霉能积累β-胡萝卜素，而突变株C2无法合成类胡萝卜素，突变株C9则积累番茄红素。含有比例为p的C2细胞核和比例为(1 - p)的C9细胞核的异核体，对于不同的p值，分别以(1 - p)、p(1 - p)和p²的相对量积累番茄红素、γ-胡萝卜素和β-胡萝卜素。结果表明，这些结果是由一种类胡萝卜素生成酶聚集体的作用所预期的，该聚集体作为一条装配线起作用，包含两个环化酶拷贝（C9菌株中该酶有缺陷）以及其他酶。
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