Baron S, Bogomolova N N, Billiau A, Levy H B, Buckler C E, Stern R, Naylor R
Proc Natl Acad Sci U S A. 1969 Sep;64(1):67-74. doi: 10.1073/pnas.64.1.67.
A study was undertaken to help determine whether synthetic nonbase-paired, single-stranded RNA as well as synthetic base-paired, double-stranded RNA could induce interferon. Synthetic polyriboinosinic acid and polyribocytidylic acid induced interferon and interferon-like resistance in several different cell culture systems. However, in the intact rabbit only the most active preparations of single-stranded RNA induced circulating interferon when injected intravenously. The induced interferon and antiviral effect in tissue culture and animals was shown to have characteristics of the interferon system. Contamination of the single-stranded synthetic RNA materials by small amounts of base-paired, double-stranded RNA was ruled out by failure to find sufficient heterologous nucleic acid bases by differential activities of single-stranded and double-stranded RNA's in different cell systems and by failure to detect double-stranded RNA in poly-rI upon chromatography on benzoylated DEAE-cellulose.
开展了一项研究，以确定合成的非碱基配对单链RNA以及合成的碱基配对双链RNA是否能够诱导干扰素。合成的聚肌苷酸和聚胞苷酸在几种不同的细胞培养系统中诱导了干扰素和类似干扰素的抗性。然而，在完整的兔子中，只有活性最强的单链RNA制剂静脉注射时能诱导循环干扰素。在组织培养和动物中诱导产生的干扰素和抗病毒作用显示出具有干扰素系统的特征。通过在不同细胞系统中利用单链和双链RNA的差异活性未发现足够的异源核酸碱基，以及在苯甲酰化DEAE-纤维素上进行色谱分析时未在聚肌苷酸中检测到双链RNA，排除了单链合成RNA材料被少量碱基配对双链RNA污染的可能性。
