大鼠再生肝中DNA复制过程中DNA物理状态的变化。
Changes in the physical state of DNA during replication in regenerating liver of the rat.
作者信息
Berger H, Irvin J L
出版信息
Proc Natl Acad Sci U S A. 1970 Jan;65(1):152-9. doi: 10.1073/pnas.65.1.152.
Abstract
Evidence is presented which demonstrates that DNA synthesis in regenerating liver is discontinuous. In pulse-labeling experiments from 30 seconds to 10 minutes, the earliest detectable intermediate in DNA replication appears to be double-stranded DNA which has a significant degree of single-stranded character probably due to the existence of gaps in the newly synthesized strand. The label begins to move into completely double-stranded DNA 10-30 minutes after the commencement of labeling. The average molecular weight of the single-stranded "unit" of DNA replication is determined to be approximately 7.5 million. Smaller units may be produced but not observed as a result of imperfect synchronization of DNA synthesis.
摘要
有证据表明，再生肝脏中的DNA合成是不连续的。在30秒至10分钟的脉冲标记实验中，DNA复制中最早可检测到的中间体似乎是双链DNA，其具有显著程度的单链特征，这可能是由于新合成链中存在缺口所致。标记在标记开始后10 - 30分钟开始转移到完全双链的DNA中。DNA复制的单链“单位”的平均分子量被确定为约750万。可能会产生较小的单位，但由于DNA合成的同步性不完善而未被观察到。
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