成年大鼠肝细胞中核糖体RNA、5S RNA和转运RNA基因的利用情况。
The utilization of genes for ribosomal RNA, 5S RNA, and transfer RNA in liver cells of adult rats.
作者信息
Quincey R V, Wilson S H
出版信息
Proc Natl Acad Sci U S A. 1969 Nov;64(3):981-8. doi: 10.1073/pnas.64.3.981.
Abstract
The rates of synthesis of ribosomes, 5S RNA, and tRNA necessary to maintain the steady-state concentrations of these entities in liver cytoplasm of adult rats were determined. On the average, each liver cell in the adult rat synthesizes 650 ribosomes, 650 molecules of 5S RNA, and 11,000 molecules of tRNA each minute. The numbers of genes per liver cell for rRNA, 5S RNA, and tRNA were 330, 1660, and 13,000, respectively, as determined by RNA: DNA hybridization experiments. Thus, on the average, individual genes for rRNA, tRNA, and 5S RNA are transcribed twice a minute, once a minute, and once every 2.5 minutes, respectively, in the adult rat liver.
摘要
测定了维持成年大鼠肝细胞质中核糖体、5S RNA和tRNA稳态浓度所需的这些物质的合成速率。成年大鼠的每个肝细胞平均每分钟合成650个核糖体、650个5S RNA分子和11,000个tRNA分子。通过RNA:DNA杂交实验确定，每个肝细胞中rRNA、5S RNA和tRNA的基因数量分别为330、1660和13,000。因此，在成年大鼠肝脏中，rRNA、tRNA和5S RNA的单个基因平均分别每分钟转录两次、每分钟转录一次和每2.5分钟转录一次。
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