Wilson S H, Hoagland M B
Biochem J. 1967 May;103(2):556-66. doi: 10.1042/bj1030556.
Starvation of rats for several days led to marked decrease in cytoplasmic polysomes and accumulation of breakdown products having S values less than 200s. Re-feeding of the starved animals induced a rapid reassembly of polysomes. These newly formed polysomes, in the presence of actinomycin D, decayed in a biphasic fashion: about two-thirds decayed with an apparent half-life of 3-3(1/2)hr. but the other one-third were much more stable. Evidence that polysome decay is an accurate reflexion of messenger RNA stability is presented, and it is concluded that in the presence of large doses of actinomycin D, rat-liver cytoplasm contains messenger RNA classes of widely varying stability, the more stable class having a half-life of at least 80hr. The half-life of liver ribosomes was also determined and was found to be 110-127hr.
大鼠饥饿数天导致细胞质多核糖体显著减少，且出现沉降系数小于200s的降解产物积累。对饥饿动物重新喂食会诱导多核糖体快速重新组装。这些新形成的多核糖体在放线菌素D存在的情况下呈双相衰变：约三分之二以3 - 3.5小时的表观半衰期衰变，但另外三分之一则稳定得多。文中提供了多核糖体衰变是信使核糖核酸稳定性准确反映的证据，并得出结论：在大剂量放线菌素D存在的情况下，大鼠肝脏细胞质中含有稳定性差异很大的信使核糖核酸类别，较稳定的类别半衰期至少为80小时。还测定了肝脏核糖体的半衰期，发现为110 - 127小时。
